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Overview Data source: UniProt

Official Gene Symbol Other Aliases
TERF2 TRBF2,TRF2
Sequence Length (AA) Molecular Weight (Da)
542 59594
Protein Name
Telomeric repeat-binding factor 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAGAGTAGP ASGPGVVRDP AASQPRKRPG REGGEGARRS DTMAGGGGSS
60 70 80 90 100
DGSGRAAGRR ASRSSGRARR GRHEPGLGGP AERGAGEARL EEAVNRWVLK
110 120 130 140 150
FYFHEALRAF RGSRYGDFRQ IRDIMQALLV RPLGKEHTVS RLLRVMQCLS
160 170 180 190 200
RIEEGENLDC SFDMEAELTP LESAINVLEM IKTEFTLTEA VVESSRKLVK
210 220 230 240 250
EAAVIICIKN KEFEKASKIL KKHMSKDPTT QKLRNDLLNI IREKNLAHPV
260 270 280 290 300
IQNFSYETFQ QKMLRFLESH LDDAEPYLLT MAKKALKSES AASSTGKEDK
310 320 330 340 350
QPAPGPVEKP PREPARQLRN PPTTIGMMTL KAAFKTLSGA QDSEAAFAKL
360 370 380 390 400
DQKDLVLPTQ ALPASPALKN KRPRKDENES SAPADGEGGS ELQPKNKRMT
410 420 430 440 450
ISRLVLEEDS QSTEPSAGLN SSQEAASAPP SKPTVLNQPL PGEKNPKVPK
460 470 480 490 500
GKWNSSNGVE EKETWVEEDE LFQVQAAPDE DSTTNITKKQ KWTVEESEWV
510 520 530 540 542
KAGVQKYGEG NWAAISKNYP FVNRTAVMIK DRWRTMKRLG MN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-956 Collapse assay details

Data source: Panorama

Official Gene Symbol
TERF2
Peptide Modified Sequence
DLVLPTQALPAS[+80.0]PALK
Modification Type
Phospho (ST)
Protein - Site of Modification
365
Peptide - Site of Modification
12
Peptide Start
354
Peptide End
369
CPTAC ID
CPTAC-956
Peptide Molecular Mass
1,712.9114
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 46.6 25.3 22.6 87.2 32.4 30.8 98.9 41.1 38.2 15 24 15
y13 (2+) 46.7 29.7 18.4 69.8 33.7 18.4 84 44.9 26 15 24 15
y7 (1+) 44.4 16 14 66.5 25 15.7 80 29.7 21 15 24 15
sum 30 12.6 9.7 65.4 22.9 14.3 72 26.1 17.3 15 24 15

Additional Resources and Comments


Assay Details for CPTAC-3256 Collapse assay details

Data source: Panorama

Official Gene Symbol
TERF2
Peptide Sequence
DLVLPTQALPASPALK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
354
Peptide End
369
CPTAC ID
CPTAC-3256
Peptide Molecular Mass
1,632.9451
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 13.9 17.7 22.9 15.4 31.1 34.2 20.7 35.8 41.2 15 15 15
y8 (1+) 34.8 20.5 21.9 57 39.9 32.7 66.8 44.9 39.4 15 15 15
y7 (1+) 22.2 22.1 19.6 34.8 35.6 26.6 41.3 41.9 33 15 15 15
sum 13.9 15.9 20 24.1 31.7 30.8 27.8 35.5 36.7 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-3257 Collapse assay details

Data source: Panorama

Official Gene Symbol
TERF2
Peptide Modified Sequence
DLVLPTQALPAS[+80.0]PALK
Modification Type
Phospho (ST)
Protein - Site of Modification
365
Peptide - Site of Modification
12
Peptide Start
354
Peptide End
369
CPTAC ID
CPTAC-3257
Peptide Molecular Mass
1,712.9114
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 16.9 29.5 24.3 25.3 30.6 33.6 30.4 42.5 41.5 15 15 15
y13 (2+) 16.5 26.2 31 22.4 30.6 41.7 27.8 40.3 52 15 15 15
y12 (2+) 18 23 20.8 18.4 33.3 32.5 25.7 40.5 38.6 15 15 15
y7 (1+) 14.4 26 27.9 16.7 32.4 37.3 22.1 41.5 46.6 15 15 15
sum 10.9 23.9 23.5 15.3 30.5 34.3 18.8 38.7 41.6 15 15 15

Additional Resources and Comments