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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ABLIM1 ABLIM, KIAA0059, LIMAB1
Sequence Length (AA) Molecular Weight (Da)
778 87688
Protein Name
Actin-binding LIM protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPAFLGLKCL GKLCSSEKSK VTSSERTSAR GSNRKRLIVE DRRVSGTSFT
60 70 80 90 100
AHRRATITHL LYLCPKDYCP RGRVCNSVDP FVAHPQDPHH PSEKPVIHCH
110 120 130 140 150
KCGEPCKGEV LRVQTKHFHI KCFTCKVCGC DLAQGGFFIK NGEYLCTLDY
160 170 180 190 200
QRMYGTRCHG CGEFVEGEVV TALGKTYHPN CFACTICKRP FPPGDRVTFN
210 220 230 240 250
GRDCLCQLCA QPMSSSPKET TFSSNCAGCG RDIKNGQALL ALDKQWHLGC
260 270 280 290 300
FKCKSCGKVL TGEYISKDGA PYCEKDYQGL FGVKCEACHQ FITGKVLEAG
310 320 330 340 350
DKHYHPSCAR CSRCNQMFTE GEEMYLQGST VWHPDCKQST KTEEKLRPTR
360 370 380 390 400
TSSESIYSRP GSSIPGSPGH TIYAKVDNEI LDYKDLAAIP KVKAIYDIER
410 420 430 440 450
PDLITYEPFY TSGYDDKQER QSLGESPRTL SPTPSAEGYQ DVRDRMIHRS
460 470 480 490 500
TSQGSINSPV YSRHSYTPTT SRSPQHFHRP GNEPSSGRNS PLPYRPDSRP
510 520 530 540 550
LTPTYAQAPK HFHVPDQGIN IYRKPPIYKQ HAALAAQSKS SEDIIKFSKF
560 570 580 590 600
PAAQAPDPSE TPKIETDHWP GPPSFAVVGP DMKRRSSGRE EDDEELLRRR
610 620 630 640 650
QLQEEQLMKL NSGLGQLILK EEMEKESRER SSLLASRYDS PINSASHIPS
660 670 680 690 700
SKTASLPGYG RNGLHRPVST DFAQYNSYGD VSGGVRDYQT LPDGHMPAMR
710 720 730 740 750
MDRGVSMPNM LEPKIFPYEM LMVTNRGRNK ILREVDRTRL ERHLAPEVFR
760 770 778
EIFGMSIQEF DRLPLWRRND MKKKAKLF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-957 Collapse assay details

Data source: Panorama

Official Gene Symbol
ABLIM1
Peptide Modified Sequence
STS[+80.0]QGSINSPVYSR
Modification Type
Phospho (ST)
Protein - Site of Modification
452
Peptide - Site of Modification
3
Peptide Start
450
Peptide End
463
CPTAC ID
CPTAC-957
Peptide Molecular Mass
1,561.6774
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12-98 (2+) 13.5 7.7 7.5 19.1 7.3 8.6 23.4 10.6 11.4 15 24 15
y5 (1+) 17.2 8 9.5 29.2 7.6 10.9 33.9 11 14.5 15 24 15
y7 (1+) 14.1 5.7 10.1 23.5 5.9 11.7 27.4 8.2 15.5 15 24 15
y9 (1+) 17.7 7 9.9 34.9 7.5 8.4 39.1 10.3 13 15 24 15
sum 13.5 6.3 7.8 23.2 6.1 9 26.8 8.8 11.9 15 24 15


Additional Resources and Comments