UBE2T, CPTAC-3263 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
UBE2T	N/A

Sequence Length (AA)	Molecular Weight (Da)
197	22521

Protein Name
Ubiquitin-conjugating enzyme E2 T

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MQRASRLKRE	LHMLATEPPP	GITCWQDKDQ	MDDLRAQILG	GANTPYEKGV
60	70	80	90	100
FKLEVIIPER	YPFEPPQIRF	LTPIYHPNID	SAGRICLDVL	KLPPKGAWRP
110	120	130	140	150
SLNIATVLTS	IQLLMSEPNP	DDPLMADISS	EFKYNKPAFL	KNARQWTEKH
160	170	180	190	197
ARQKQKADEE	EMLDNLPEAG	DSRVHNSTQK	RKASQLVGIE	KKFHPDV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3262 Collapse assay details

Data source: Panorama

Official Gene Symbol: UBE2T
Peptide Sequence: ASQLVGIEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 183
Peptide End: 191

CPTAC ID: CPTAC-3262
Peptide Molecular Mass: 943.5338
Species: Homo sapiens (Human)
Assay Type: Enrichment ImmunoMRM
Enrichment Method: peptide immunoaffinity
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6500 QTRAP (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Ultra NanoLC 2D+ (Eksigent)
Column Packing: ReproSil-Pur C18-AQ 3um
Column Dimensions: 100 x 0.075 mm
Flow Rate: 300 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	9.7	8.7	6.4	10.7	12.7	7.2	14.4	15.4	9.6	15	15	15
y5 (1+)	12.7	6.2	5.7	13.1	13.9	7.8	18.2	15.2	9.7	15	15	15
y6 (1+)	10.6	8.1	6.3	11.8	11.5	8	15.9	14.1	10.2	15	15	15
y4 (1+)	9.3	5.8	10.4	9.4	7.9	11	13.2	9.8	15.1	15	15	15
sum	4.3	4.7	4.8	6.3	10.1	6.8	7.6	11.1	8.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments