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Overview Data source: UniProt

Official Gene Symbol Other Aliases
UBE2T N/A
Sequence Length (AA) Molecular Weight (Da)
197 22521
Protein Name
Ubiquitin-conjugating enzyme E2 T
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV
60 70 80 90 100
FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP
110 120 130 140 150
SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH
160 170 180 190 197
ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3262 Collapse assay details

Data source: Panorama

Official Gene Symbol
UBE2T
Peptide Sequence
ASQLVGIEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
183
Peptide End
191
CPTAC ID
CPTAC-3262
Peptide Molecular Mass
943.5338
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 9.7 8.7 6.4 10.7 12.7 7.2 14.4 15.4 9.6 15 15 15
y5 (1+) 12.7 6.2 5.7 13.1 13.9 7.8 18.2 15.2 9.7 15 15 15
y6 (1+) 10.6 8.1 6.3 11.8 11.5 8 15.9 14.1 10.2 15 15 15
y4 (1+) 9.3 5.8 10.4 9.4 7.9 11 13.2 9.8 15.1 15 15 15
sum 4.3 4.7 4.8 6.3 10.1 6.8 7.6 11.1 8.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-3263 Collapse assay details

Data source: Panorama

Official Gene Symbol
UBE2T
Peptide Modified Sequence
AS[+80.0]QLVGIEK
Modification Type
Phospho (ST)
Protein - Site of Modification
184
Peptide - Site of Modification
2
Peptide Start
183
Peptide End
191
CPTAC ID
CPTAC-3263
Peptide Molecular Mass
1,023.5002
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 21.3 13 11.3 24.5 12.5 11.1 32.5 18 15.8 15 15 15
y6 (1+) 28.3 12.3 14 40.7 17.3 12.1 49.6 21.2 18.5 15 15 15
y5 (1+) 24.5 10.3 13 29.1 13.1 12.3 38 16.7 17.9 15 15 15
sum 13.5 7.7 8.5 18.4 8 8.7 22.8 11.1 12.2 15 15 15

Additional Resources and Comments