CCT8, CPTAC-331 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CCT8	C21orf112, CCTQ, KIAA0002

Sequence Length (AA)	Molecular Weight (Da)
548	59621

Protein Name
T-complex protein 1 subunit theta

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MALHVPKAPG	FAQMLKEGAK	HFSGLEEAVY	RNIQACKELA	QTTRTAYGPN
60	70	80	90	100
GMNKMVINHL	EKLFVTNDAA	TILRELEVQH	PAAKMIVMAS	HMQEQEVGDG
110	120	130	140	150
TNFVLVFAGA	LLELAEELLR	IGLSVSEVIE	GYEIACRKAH	EILPNLVCCS
160	170	180	190	200
AKNLRDIDEV	SSLLRTSIMS	KQYGNEVFLA	KLIAQACVSI	FPDSGHFNVD
210	220	230	240	250
NIRVCKILGS	GISSSSVLHG	MVFKKETEGD	VTSVKDAKIA	VYSCPFDGMI
260	270	280	290	300
TETKGTVLIK	TAEELMNFSK	GEENLMDAQV	KAIADTGANV	VVTGGKVADM
310	320	330	340	350
ALHYANKYNI	MLVRLNSKWD	LRRLCKTVGA	TALPRLTPPV	LEEMGHCDSV
360	370	380	390	400
YLSEVGDTQV	VVFKHEKEDG	AISTIVLRGS	TDNLMDDIER	AVDDGVNTFK
410	420	430	440	450
VLTRDKRLVP	GGGATEIELA	KQITSYGETC	PGLEQYAIKK	FAEAFEAIPR
460	470	480	490	500
ALAENSGVKA	NEVISKLYAV	HQEGNKNVGL	DIEAEVPAVK	DMLEAGILDT
510	520	530	540	548
YLGKYWAIKL	ATNAAVTVLR	VDQIIMAKPA	GGPKPPSGKK	DWDDDQND

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-332 Collapse assay details

Data source: Panorama

Official Gene Symbol: CCT8
Peptide Sequence: AIADTGANVVVTGGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 282
Peptide End: 296

CPTAC ID: CPTAC-332
Peptide Molecular Mass: 1,371.7358
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Broad Institute and FHCRC
Submitting Lab PI: Steve Carr

Publication

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Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y13 (1+)	5.5	5.6	7.1	6.9	7.2	10.2	8.8	9.1	12.4	15	25	15
y10 (1+)	3.9	7.2	5.4	6.7	7.8	7.4	7.8	10.6	9.2	15	25	15
y8 (1+)	9.2	6	3.9	12.5	6.5	8.9	15.5	8.8	9.7	15	25	15
sum	4.3	5.8	5	7.3	6.8	7.1	8.5	8.9	8.7	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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