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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CBR1 CBR, CRN, SDR21C1
Sequence Length (AA) Molecular Weight (Da)
277 30375
Protein Name
Carbonyl reductase [NADPH] 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSSGIHVALV TGGNKGIGLA IVRDLCRLFS GDVVLTARDV TRGQAAVQQL
60 70 80 90 100
QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLDVLVNN AGIAFKVADP
110 120 130 140 150
TPFHIQAEVT MKTNFFGTRD VCTELLPLIK PQGRVVNVSS IMSVRALKSC
160 170 180 190 200
SPELQQKFRS ETITEEELVG LMNKFVEDTK KGVHQKEGWP SSAYGVTKIG
210 220 230 240 250
VTVLSRIHAR KLSEQRKGDK ILLNACCPGW VRTDMAGPKA TKSPEEGAET
260 270 277
PVYLALLPPD AEGPHGQFVS EKRVEQW

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-330 Collapse assay details

Data source: Panorama

Official Gene Symbol
CBR1
Peptide Modified Sequence
ILLNAC[+57.0]C[+57.0]PGWVR
Modification Type
Carbamidomethyl Cysteine, Carbamidomethyl Cysteine
Protein - Site of Modification
226, 227
Peptide - Site of Modification
6, 7
Peptide Start
221
Peptide End
232
CPTAC ID
CPTAC-330
Peptide Molecular Mass
1,457.7272
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 11.2 8.2 6.3 11.3 9.5 9.1 15.9 12.5 11.1 15 25 15
y8 (1+) 15 8.1 8.5 15.9 11.1 6.5 21.9 13.7 10.7 15 25 15
y7 (1+) 14.7 8.6 5.9 10.2 10.2 6.5 17.9 13.3 8.8 15 25 15
sum 9.9 6.7 5.7 8.3 9.2 5.1 12.9 11.4 7.6 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-329 Collapse assay details

Data source: Panorama

Official Gene Symbol
CBR1
Peptide Sequence
LFSGDVVLTAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
28
Peptide End
38
CPTAC ID
CPTAC-329
Peptide Molecular Mass
1,176.6503
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 5.9 8 4.9 9.2 13.1 13.3 10.9 15.3 14.2 15 25 15
y8 (1+) 8.6 13.5 10.7 9 16.3 14.1 12.4 21.2 17.7 15 25 15
y6 (1+) 17.5 21.5 8.6 23.8 26.7 23.5 29.5 34.3 25 15 25 15
sum 6.7 8.4 5.3 9 13.4 13 11.2 15.8 14 15 25 15


Additional Resources and Comments


Assay Details for non-CPTAC-3775 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cbr1
Peptide Sequence
FSGDVVLAAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
29
Peptide End
38
CPTAC ID
non-CPTAC-3775
Peptide Molecular Mass
1,033.5556
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 8.4 8.1 8.9 8.8 13.3 12.8 12.2 15.6 15.6 15 15 15
y4 (1+) 8.7 4.4 5 10.4 6.3 8.1 13.6 7.7 9.5 15 15 15
y5 (1+) 8.8 4.3 3.7 10.6 8.2 7.5 13.8 9.3 8.4 15 15 15
y8 (1+) 7.8 3.4 6.2 12.7 7.1 7.9 14.9 7.9 10 15 15 15
y9 (1+) 9.6 5.4 4.7 10.2 9 8.8 14 10.5 10 15 15 15
sum 3.7 2 2.1 7.2 7.1 7.2 8.1 7.4 7.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3416 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cbr1
Peptide Sequence
TNFFGTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
113
Peptide End
119
CPTAC ID
non-CPTAC-3416
Peptide Molecular Mass
841.4083
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.7 4.6 4.8 10.8 8.7 5 12.2 9.8 6.9 15 14 15
y3 (1+) 5.4 2.7 3.3 12.2 7.7 6.3 13.3 8.2 7.1 15 14 15
y4 (1+) 4 3.3 3.4 14.1 7.2 4.5 14.7 7.9 5.6 15 14 15
y5 (1+) 4 4.1 3.2 13.6 5.8 4.1 14.2 7.1 5.2 15 14 15
y6 (1+) 11.6 6.4 7.2 19.7 9.6 7.8 22.9 11.5 10.6 15 14 15
sum 1.7 1.5 1.3 12.8 6.2 3.2 12.9 6.4 3.5 15 14 15


Additional Resources and Comments