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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
HNRNPM HNRPM, NAGR1
Sequence Length (AA) Molecular Weight (Da)
730 77516
Protein Name
Heterogeneous nuclear ribonucleoprotein M
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAGVEAAAE VAATEIKMEE ESGAPGVPSG NGAPGPKGEG ERPAQNEKRK
60 70 80 90 100
EKNIKRGGNR FEPYANPTKR YRAFITNIPF DVKWQSLKDL VKEKVGEVTY
110 120 130 140 150
VELLMDAEGK SRGCAVVEFK MEESMKKAAE VLNKHSLSGR PLKVKEDPDG
160 170 180 190 200
EHARRAMQKV MATTGGMGMG PGGPGMITIP PSILNNPNIP NEIIHALQAG
210 220 230 240 250
RLGSTVFVAN LDYKVGWKKL KEVFSMAGVV VRADILEDKD GKSRGIGTVT
260 270 280 290 300
FEQSIEAVQA ISMFNGQLLF DRPMHVKMDE RALPKGDFFP PERPQQLPHG
310 320 330 340 350
LGGIGMGLGP GGQPIDANHL NKGIGMGNIG PAGMGMEGIG FGINKMGGME
360 370 380 390 400
GPFGGGMENM GRFGSGMNMG RINEILSNAL KRGEIIAKQG GGGGGGSVPG
410 420 430 440 450
IERMGPGIDR LGGAGMERMG AGLGHGMDRV GSEIERMGLV MDRMGSVERM
460 470 480 490 500
GSGIERMGPL GLDHMASSIE RMGQTMERIG SGVERMGAGM GFGLERMAAP
510 520 530 540 550
IDRVGQTIER MGSGVERMGP AIERMGLSME RMVPAGMGAG LERMGPVMDR
560 570 580 590 600
MATGLERMGA NNLERMGLER MGANSLERMG LERMGANSLE RMGPAMGPAL
610 620 630 640 650
GAGIERMGLA MGGGGGASFD RAIEMERGNF GGSFAGSFGG AGGHAPGVAR
660 670 680 690 700
KACQIFVRNL PFDFTWKMLK DKFNECGHVL YADIKMENGK SKGCGVVKFE
710 720 730
SPEVAERACR MMNGMKLSGR EIDVRIDRNA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-385 Collapse assay details

Data source: Panorama

Official Gene Symbol
HNRNPM
Peptide Sequence
AFITNIPFDVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
73
Peptide End
83
CPTAC ID
CPTAC-385
Peptide Molecular Mass
1,263.6863
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 15.6 9 4.8 22.2 14.8 9.1 27.1 17.3 10.3 25 15 15
y8 (1+) 6 4.9 3.5 8.5 8 5.9 10.4 9.4 6.9 25 15 15
y7 (1+) 6.6 5.3 4.6 7.7 7.6 6.2 10.1 9.3 7.7 25 15 15
sum 6 4.2 2.3 7 6.2 5.9 9.2 7.5 6.3 25 15 15


Additional Resources and Comments


Assay Details for CPTAC-386 Collapse assay details

Data source: Panorama

Official Gene Symbol
HNRNPM
Peptide Sequence
LGSTVFVANLDYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
202
Peptide End
214
CPTAC ID
CPTAC-386
Peptide Molecular Mass
1,425.7504
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 16.1 9 3.7 19.9 10.5 13.6 25.6 13.8 14.1 15 25 15
y7 (1+) 16.6 7.9 4.7 21.6 12.8 12.9 27.2 15 13.7 15 25 15
y6 (1+) 23 8.5 3.4 30.6 14 11.6 38.3 16.4 12.1 15 25 15
sum 12.8 7.1 3.2 18 10.9 12.7 22.1 13 13.1 15 25 15


Additional Resources and Comments