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Overview Data source: UniProt

Official Gene Symbol Other Aliases
HRSP12 PSP
Sequence Length (AA) Molecular Weight (Da)
137 14494
Protein Name
Ribonuclease UK114
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG
60 70 80 90 100
VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF
110 120 130 137
KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-387 Collapse assay details

Data source: Panorama

Official Gene Symbol
HRSP12
Peptide Modified Sequence
AAGC[+57.0]DFTNVVK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
71
Peptide - Site of Modification
4
Peptide Start
68
Peptide End
78
CPTAC ID
CPTAC-387
Peptide Molecular Mass
1,180.5547
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 6.8 6.6 6.5 6.9 9 8.9 9.7 11.2 11 15 25 15
y7 (1+) 6.2 6.2 3.7 7.8 7.6 8.3 10 9.8 9.1 15 25 15
y6 (1+) 6.1 9.6 10.3 13.8 11.8 14.7 15.1 15.2 17.9 15 25 15
sum 5.1 6 4.6 7.3 7.4 8.8 8.9 9.5 9.9 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-388 Collapse assay details

Data source: Panorama

Official Gene Symbol
HRSP12
Peptide Sequence
AAYQVAALPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
108
Peptide End
117
CPTAC ID
CPTAC-388
Peptide Molecular Mass
1,030.5811
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7.1 5.3 6.9 9.8 7.7 10 12.1 9.3 12.1 15 25 15
y7 (1+) 5.7 8.8 4.9 7.1 10.8 7.1 9.1 13.9 8.6 15 25 15
y6 (1+) 7.1 7.5 5.6 9 8.5 6.9 11.5 11.3 8.9 15 25 15
sum 5.1 6.7 3.5 7.1 8.7 5.5 8.7 11 6.5 15 25 15

Additional Resources and Comments