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Overview Data source: UniProt

Official Gene Symbol Other Aliases
HSPD1 HSP60
Sequence Length (AA) Molecular Weight (Da)
573 61055
Protein Name
60 kDa heat shock protein, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLRLPTVFRQ MRPVSRVLAP HLTRAYAKDV KFGADARALM LQGVDLLADA
60 70 80 90 100
VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQD
110 120 130 140 150
VANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA
160 170 180 190 200
VIAELKKQSK PVTTPEEIAQ VATISANGDK EIGNIISDAM KKVGRKGVIT
210 220 230 240 250
VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK
260 270 280 290 300
ISSIQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV
310 320 330 340 350
KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPH DLGKVGEVIV
360 370 380 390 400
TKDDAMLLKG KGDKAQIEKR IQEIIEQLDV TTSEYEKEKL NERLAKLSDG
410 420 430 440 450
VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA
460 470 480 490 500
LDSLTPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKIMQSSSE
510 520 530 540 550
VGYDAMAGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEVVVTEIP
560 570 573
KEEKDPGMGA MGGMGGGMGG GMF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-392 Collapse assay details

Data source: Panorama

Official Gene Symbol
HSPD1
Peptide Modified Sequence
AAVEEGIVLGGGC[+57.0]ALLR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
442
Peptide - Site of Modification
13
Peptide Start
430
Peptide End
446
CPTAC ID
CPTAC-392
Peptide Molecular Mass
1,683.8978
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 23 10.4 7.6 27.2 15 16.7 35.6 18.3 18.3 15 25 15
y10 (1+) 8.2 8.5 6.6 18.2 13.7 17.3 20 16.1 18.5 15 25 15
y9 (1+) 12.8 8.4 5.6 20.5 14.6 17.3 24.2 16.8 18.2 15 25 15
sum 9.3 7.9 6 18.3 14 17.1 20.5 16.1 18.1 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-391 Collapse assay details

Data source: Panorama

Official Gene Symbol
HSPD1
Peptide Sequence
LVQDVANNTNEEAGDGTTTATVLAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
97
Peptide End
121
CPTAC ID
CPTAC-391
Peptide Molecular Mass
2,559.2413
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 9.2 5.8 4.3 9.3 5.2 9.1 13.1 7.8 10.1 25 15 15
y20 (2+) 20.1 11.6 6.5 20.2 11.7 7.7 28.5 16.5 10.1 25 15 15
y19 (2+) 26.3 14.3 4.8 27.7 12.6 9.1 38.2 19.1 10.3 25 15 15
sum 6.3 4.1 3.1 6.6 4.5 8.1 9.1 6.1 8.7 25 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3305 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hspd1
Peptide Sequence
GIIDPTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
517
Peptide End
523
CPTAC ID
non-CPTAC-3305
Peptide Molecular Mass
742.4225
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 7.9 4.4 3.3 12 6.6 5.7 14.4 7.9 6.6 15 15 15
y6 (2+) 46.6 27.8 15.1 46 29.1 22.5 65.5 40.2 27.1 15 15 15
y2 (1+) 18.3 3.6 4.1 18.4 6 7.5 26 7 8.5 15 15 15
y4 (1+) 10.7 4.2 5.5 18.4 9.4 5.6 21.3 10.3 7.8 15 15 15
y5 (1+) 4.4 3 3.2 13.6 4.6 4.8 14.3 5.5 5.8 15 15 15
sum 3.7 2.2 1.8 12.7 5 3.5 13.2 5.5 3.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3304 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hspd1
Peptide Sequence
NAGVEGSLIVEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
482
Peptide End
493
CPTAC ID
non-CPTAC-3304
Peptide Molecular Mass
1,214.6507
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 5.1 5.2 6.4 15.6 9 7 16.4 10.4 9.5 15 15 15
y7 (1+) 5.3 3.9 6.2 17.9 6.6 6.1 18.7 7.7 8.7 15 15 15
b4 (1+) 6.9 5.6 4.5 12.6 8.6 4.5 14.4 10.3 6.4 15 15 15
y10 (1+) 8.9 3.7 3.8 15.3 9.9 4.5 17.7 10.6 5.9 15 15 15
y8 (1+) 6.5 3.6 4 15.3 8.7 7.5 16.6 9.4 8.5 15 15 15
sum 3 1.6 2.2 14.6 8.2 3.9 14.9 8.4 4.5 15 15 15

Additional Resources and Comments