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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CCT6A CCT6,CCTZ
Sequence Length (AA) Molecular Weight (Da)
531 58024
Protein Name
T-complex protein 1 subunit zeta
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAVKTLNPK AEVARAQAAL AVNISAARGL QDVLRTNLGP KGTMKMLVSG
60 70 80 90 100
AGDIKLTKDG NVLLHEMQIQ HPTASLIAKV ATAQDDITGD GTTSNVLIIG
110 120 130 140 150
ELLKQADLYI SEGLHPRIIT EGFEAAKEKA LQFLEEVKVS REMDRETLID
160 170 180 190 200
VARTSLRTKV HAELADVLTE AVVDSILAIK KQDEPIDLFM IEIMEMKHKS
210 220 230 240 250
ETDTSLIRGL VLDHGARHPD MKKRVEDAYI LTCNVSLEYE KTEVNSGFFY
260 270 280 290 300
KSAEEREKLV KAERKFIEDR VKKIIELKRK VCGDSDKGFV VINQKGIDPF
310 320 330 340 350
SLDALSKEGI VALRRAKRRN MERLTLACGG VALNSFDDLS PDCLGHAGLV
360 370 380 390 400
YEYTLGEEKF TFIEKCNNPR SVTLLIKGPN KHTLTQIKDA VRDGLRAVKN
410 420 430 440 450
AIDDGCVVPG AGAVEVAMAE ALIKHKPSVK GRAQLGVQAF ADALLIIPKV
460 470 480 490 500
LAQNSGFDLQ ETLVKIQAEH SESGQLVGVD LNTGEPMVAA EVGVWDNYCV
510 520 530 531
KKQLLHSCTV IATNILLVDE IMRAGMSSLK G

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-41 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCT6A
Peptide Sequence
ALQFLEEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
130
Peptide End
138
CPTAC ID
CPTAC-41
Peptide Molecular Mass
1,075.5914
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 16.5 9.5 9.1 25.8 12.3 6.8 30.6 15.5 11.4 15 25 15
y6 (1+) 11.5 7 4.9 13.5 8.3 4.6 17.7 10.9 6.7 15 25 15
y5 (1+) 22.4 9 6.2 23.7 9.9 11.6 32.6 13.4 13.2 15 25 15
sum 12.2 7.5 5.6 14.6 8.9 4.5 19 11.6 7.2 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-42 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCT6A
Peptide Sequence
VLAQNSGFDLQETLVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
450
Peptide End
465
CPTAC ID
CPTAC-42
Peptide Molecular Mass
1,760.9309
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 11.3 7.4 4.7 28.5 9.7 13.7 30.7 12.2 14.5 15 25 15
y10 (1+) 12.2 7 7.4 29.5 8.4 12.8 31.9 10.9 14.8 15 25 15
y8 (1+) 13.1 7.5 11.6 15.8 9.7 11.5 20.5 12.3 16.3 15 25 15
sum 8.7 6 5.7 23.8 8.1 11.5 25.3 10.1 12.8 15 25 15

Additional Resources and Comments