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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CD59 MIC11,MIN1,MIN2,MIN3,MSK21
Sequence Length (AA) Molecular Weight (Da)
128 14177
Protein Name
CD59 glycoprotein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGIQGGSVLF GLLLVLAVFC HSGHSLQCYN CPNPTADCKT AVNCSSDFDA
60 70 80 90 100
CLITKAGLQV YNKCWKFEHC NFNDVTTRLR ENELTYYCCK KDLCNFNEQL
110 120 128
ENGGTSLSEK TVLLLVTPFL AAAWSLHP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-44 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD59
Peptide Modified Sequence
ENELTYYC[+57.0]C[+57.0]K
Modification Type
Carbamidomethyl Cysteine, Carbamidomethyl Cysteine
Protein - Site of Modification
88, 89
Peptide - Site of Modification
8, 9
Peptide Start
81
Peptide End
90
CPTAC ID
CPTAC-44
Peptide Molecular Mass
1,378.5533
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 46.1 22.4 22.2 66.2 25.5 25.8 80.7 33.9 34 15 25 15
y6 (1+) 35.9 11.3 18.3 56.6 13.1 23.3 67 17.3 29.6 15 25 15
y5 (1+) 42.4 13.6 19.2 64.5 13.9 18.6 77.2 19.4 26.7 15 25 15
sum 35.7 9.2 14.5 58.3 11.6 16.5 68.4 14.8 22 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-43 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD59
Peptide Modified Sequence
FEHC[+57.0]NFNDVTTR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
70
Peptide - Site of Modification
4
Peptide Start
67
Peptide End
78
CPTAC ID
CPTAC-43
Peptide Molecular Mass
1,538.6572
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 10.3 13.6 7.3 12.8 14 13.8 16.4 19.5 15.6 15 25 15
y8 (1+) 22.6 12.9 12 19.4 11.8 11.4 29.8 17.5 16.6 15 25 15
y7 (1+) 23.1 20.3 22 29.8 23.4 31.8 37.7 31 38.7 15 25 15
sum 11.1 9.4 9.5 13.4 10.6 18.1 17.4 14.2 20.4 15 25 15

Additional Resources and Comments