CD59, CPTAC-44 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CD59	MIC11, MIN1, MIN2, MIN3, MSK21

Sequence Length (AA)	Molecular Weight (Da)
128	14177

Protein Name
CD59 glycoprotein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGIQGGSVLF	GLLLVLAVFC	HSGHSLQCYN	CPNPTADCKT	AVNCSSDFDA
60	70	80	90	100
CLITKAGLQV	YNKCWKFEHC	NFNDVTTRLR	ENELTYYCCK	KDLCNFNEQL
110	120	128
ENGGTSLSEK	TVLLLVTPFL	AAAWSLHP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-44 Collapse assay details

Data source: Panorama

Official Gene Symbol: CD59
Peptide Modified Sequence: ENELTYYC[+57.0]C[+57.0]K
Modification Type: Carbamidomethyl Cysteine, Carbamidomethyl Cysteine
Protein - Site of Modification: 88, 89
Peptide - Site of Modification: 8, 9
Peptide Start: 81
Peptide End: 90

CPTAC ID: CPTAC-44
Peptide Molecular Mass: 1,378.5533
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	46.1	22.4	22.2	66.2	25.5	25.8	80.7	33.9	34	15	25	15
y6 (1+)	35.9	11.3	18.3	56.6	13.1	23.3	67	17.3	29.6	15	25	15
y5 (1+)	42.4	13.6	19.2	64.5	13.9	18.6	77.2	19.4	26.7	15	25	15
sum	35.7	9.2	14.5	58.3	11.6	16.5	68.4	14.8	22	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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