Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TAGLN SM22,WS3-10
Sequence Length (AA) Molecular Weight (Da)
201 22611
Protein Name
Transgelin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MANKGPSYGM SREVQSKIEK KYDEELEERL VEWIIVQCGP DVGRPDRGRL
60 70 80 90 100
GFQVWLKNGV ILSKLVNSLY PDGSKPVKVP ENPPSMVFKQ MEQVAQFLKA
110 120 130 140 150
AEDYGVIKTD MFQTVDLFEG KDMAAVQRTL MALGSLAVTK NDGHYRGDPN
160 170 180 190 200
WFMKKAQEHK REFTESQLQE GKHVIGLQMG SNRGASQAGM TGYGRPRQII
201
S

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-443 Collapse assay details

Data source: Panorama

Official Gene Symbol
TAGLN
Peptide Sequence
EFTESQLQEGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
162
Peptide End
172
CPTAC ID
CPTAC-443
Peptide Molecular Mass
1,294.6041
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 11.6 10.1 9.5 11.7 12.5 13.9 16.5 16.1 16.8 15 25 15
y8 (1+) 8.1 11.4 16.3 14.7 13.8 21.1 16.8 17.9 26.7 15 25 15
y7 (1+) 10.4 11.6 8.2 12.3 14 8.4 16.1 18.2 11.7 15 25 15
sum 6.2 8.1 6 8.3 10.6 7.2 10.4 13.3 9.4 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-726 Collapse assay details

Data source: Panorama

Official Gene Symbol
TAGLN
Peptide Sequence
AAEDYGVIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
100
Peptide End
108
CPTAC ID
CPTAC-726
Peptide Molecular Mass
964.4866
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 24.3 5.9 1.6 25.2 5.9 3.8 35 8.3 4.1 15 15 15
y7 (1+) 18.2 4.2 1.5 20 5.1 3.1 27 6.6 3.4 15 15 15
y8 (1+) 24.8 6.5 1.7 29.3 7.3 3 38.4 9.8 3.4 15 15 15
sum 18.4 3.6 1.3 19.6 4.5 3.2 26.9 5.8 3.5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3751 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tagln
Peptide Sequence
AAEDYGVIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
100
Peptide End
108
CPTAC ID
non-CPTAC-3751
Peptide Molecular Mass
964.4866
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 49.9 10.7 8.7 61.7 11.7 6.5 79.4 15.9 10.9 15 15 15
y2 (1+) 27 24.9 11 39.3 25.9 24.3 47.7 35.9 26.7 15 15 15
y5 (1+) 53.5 9.9 12 51.6 13.5 16.7 74.3 16.7 20.6 15 15 15
y6 (1+) 26.7 10.5 6.5 34.8 14 14.9 43.9 17.5 16.3 15 15 15
y7 (1+) 10.3 9.4 5.6 12.2 8.3 9.3 16 12.5 10.9 15 15 15
sum 17 5.6 3.6 26.1 6.5 8.3 31.1 8.6 9 15 15 15

Additional Resources and Comments