AGR2, CPTAC-456 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
AGR2	AG2

Sequence Length (AA)	Molecular Weight (Da)
175	19979

Protein Name
Anterior gradient protein 2 homolog

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEKIPVSAFL	LLVALSYTLA	RDTTVKPGAK	KDTKDSRPKL	PQTLSRGWGD
60	70	80	90	100
QLIWTQTYEE	ALYKSKTSNK	PLMIIHHLDE	CPHSQALKKV	FAENKEIQKL
110	120	130	140	150
AEQFVLLNLV	YETTDKHLSP	DGQYVPRIMF	VDPSLTVRAD	ITGRYSNRLY
160	170	175
AYEPADTALL	LDNMKKALKL	LKTEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1293 Collapse assay details

Data source: Panorama

Official Gene Symbol: AGR2
Peptide Sequence: HLSPDGQYVPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 117
Peptide End: 127

CPTAC ID: CPTAC-1293
Peptide Molecular Mass: 1,267.6309
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human serum digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b6 (1+)	18.5	15	16.3	19.9	14.6	11.6	27.2	20.9	20	15	15	15
b3 (1+)	18.6	6.3	5	24.2	7	5.3	30.5	9.4	7.3	15	15	15
y4 (1+)	11.3	4.1	5.9	18.1	4.6	6.4	21.3	6.2	8.7	15	15	15
sum	11.8	4.2	4.5	18.4	4.9	4.4	21.9	6.5	6.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-456 Collapse assay details

Data source: Panorama

Official Gene Symbol: AGR2
Peptide Sequence: HLSPDGQYVPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 117
Peptide End: 127

CPTAC ID: CPTAC-456
Peptide Molecular Mass: 1,267.6309
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI: Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.