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Overview Data source: UniProt

Official Gene Symbol Other Aliases
AGR2 AG2
Sequence Length (AA) Molecular Weight (Da)
175 19979
Protein Name
Anterior gradient protein 2 homolog
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEKIPVSAFL LLVALSYTLA RDTTVKPGAK KDTKDSRPKL PQTLSRGWGD
60 70 80 90 100
QLIWTQTYEE ALYKSKTSNK PLMIIHHLDE CPHSQALKKV FAENKEIQKL
110 120 130 140 150
AEQFVLLNLV YETTDKHLSP DGQYVPRIMF VDPSLTVRAD ITGRYSNRLY
160 170 175
AYEPADTALL LDNMKKALKL LKTEL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1293 Collapse assay details

Data source: Panorama

Official Gene Symbol
AGR2
Peptide Sequence
HLSPDGQYVPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
117
Peptide End
127
CPTAC ID
CPTAC-1293
Peptide Molecular Mass
1,267.6309
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 18.5 15 16.3 19.9 14.6 11.6 27.2 20.9 20 15 15 15
b3 (1+) 18.6 6.3 5 24.2 7 5.3 30.5 9.4 7.3 15 15 15
y4 (1+) 11.3 4.1 5.9 18.1 4.6 6.4 21.3 6.2 8.7 15 15 15
sum 11.8 4.2 4.5 18.4 4.9 4.4 21.9 6.5 6.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-456 Collapse assay details

Data source: Panorama

Official Gene Symbol
AGR2
Peptide Sequence
HLSPDGQYVPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
117
Peptide End
127
CPTAC ID
CPTAC-456
Peptide Molecular Mass
1,267.6309
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 5.1 6.4 4.7 10.1 7.9 13.1 11.3 10.2 13.9 15 25 15
y8 (1+) 8.9 7 4 13.3 7.8 10.9 16 10.5 11.6 15 25 15
y6 (1+) 19.3 6.2 4.4 37.6 10.2 12 42.3 11.9 12.8 15 25 15
sum 7.4 6.2 4.4 15.4 7.7 12.2 17.1 9.9 13 15 25 15

Additional Resources and Comments