Overview Data source: UniProt

Official Gene Symbol Other Aliases
COMT N/A
Sequence Length (AA) Molecular Weight (Da)
271 30037
Protein Name
Catechol O-methyltransferase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPEAPPLLLA AVLLGLVLLV VLLLLLRHWG WGLCLIGWNE FILQPIHNLL
60 70 80 90 100
MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV
110 120 130 140 150
DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ
160 170 180 190 200
RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD
210 220 230 240 250
TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY
260 270 271
REVVDGLEKA IYKGPGSEAG P

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-483 Collapse assay details

Data source: Panorama

Official Gene Symbol
COMT
Peptide Modified Sequence
GTVLLADNVIC[+57.0]PGAPDFLAHVR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
223
Peptide - Site of Modification
11
Peptide Start
213
Peptide End
234
CPTAC ID
CPTAC-483
Peptide Molecular Mass
2,334.2154
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y18 (2+) 20.2 20.4 16.4 48.3 25.4 20.1 52.4 32.6 25.9 15 15 15
y17 (2+) 20.4 25.2 13.2 70.4 27 25.2 73.3 36.9 28.4 15 15 15
y16 (2+) 34.3 26.8 13.9 69.2 45.3 14.6 77.2 52.6 20.2 15 15 15
sum 19.7 20.2 7.2 60 22.1 15.9 63.2 29.9 17.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-482 Collapse assay details

Data source: Panorama

Official Gene Symbol
COMT
Peptide Modified Sequence
YLPDTLLLEEC[+57.0]GLLR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
207
Peptide - Site of Modification
11
Peptide Start
197
Peptide End
211
CPTAC ID
CPTAC-482
Peptide Molecular Mass
1,803.9441
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 20.6 18.2 10 32.6 25.5 27.3 38.6 31.3 29.1 25 15 15
y9 (1+) 12.9 16.2 8.9 29.8 27.3 24.5 32.5 31.7 26.1 25 15 15
y8 (1+) 12.7 12.1 9.4 26.1 27.1 26.5 29 29.7 28.1 25 15 15
sum 12.4 13.7 9 27.8 26.9 25.6 30.4 30.2 27.1 25 15 15

Additional Resources and Comments