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Overview Data source: UniProt

Official Gene Symbol Other Aliases
DNAJA2 CPR3, HIRIP4
Sequence Length (AA) Molecular Weight (Da)
412 45746
Protein Name
DnaJ homolog subfamily A member 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MANVADTKLY DILGVPPGAS ENELKKAYRK LAKEYHPDKN PNAGDKFKEI
60 70 80 90 100
SFAYEVLSNP EKRELYDRYG EQGLREGSGG GGGMDDIFSH IFGGGLFGFM
110 120 130 140 150
GNQSRSRNGR RRGEDMMHPL KVSLEDLYNG KTTKLQLSKN VLCSACSGQG
160 170 180 190 200
GKSGAVQKCS ACRGRGVRIM IRQLAPGMVQ QMQSVCSDCN GEGEVINEKD
210 220 230 240 250
RCKKCEGKKV IKEVKILEVH VDKGMKHGQR ITFTGEADQA PGVEPGDIVL
260 270 280 290 300
LLQEKEHEVF QRDGNDLHMT YKIGLVEALC GFQFTFKHLD GRQIVVKYPP
310 320 330 340 350
GKVIEPGCVR VVRGEGMPQY RNPFEKGDLY IKFDVQFPEN NWINPDKLSE
360 370 380 390 400
LEDLLPSRPE VPNIIGETEE VELQEFDSTR GSGGGQRREA YNDSSDEESS
410 412
SHHGPGVQCA HQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-497 Collapse assay details

Data source: Panorama

Official Gene Symbol
DNAJA2
Peptide Modified Sequence
IGLVEALC[+57.0]GFQFTFK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
280
Peptide - Site of Modification
8
Peptide Start
273
Peptide End
287
CPTAC ID
CPTAC-497
Peptide Molecular Mass
1,728.8909
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 15.5 16.1 18 54.2 50.5 46.9 56.4 53 50.2 25 15 15
y9 (1+) 19.4 11.5 14.4 51 50.4 44.2 54.6 51.7 46.5 25 15 15
y8 (1+) 18.1 13.6 12.2 51.9 51.5 45.4 55 53.3 47 25 15 15
sum 15.6 11.3 14.3 52 50.7 45.2 54.3 51.9 47.4 25 15 15

Additional Resources and Comments


Assay Details for CPTAC-496 Collapse assay details

Data source: Panorama

Official Gene Symbol
DNAJA2
Peptide Sequence
VSLEDLYNGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
122
Peptide End
131
CPTAC ID
CPTAC-496
Peptide Molecular Mass
1,136.5714
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 16.3 8.4 7 24.8 10.8 7.2 29.7 13.7 10 15 25 15
y7 (1+) 15.5 9.9 6.1 20.1 10.8 4.7 25.4 14.7 7.7 15 25 15
y6 (1+) 32.9 6.7 13 26.2 10.7 14.3 42.1 12.6 19.3 15 25 15
sum 15.6 6.7 5.5 13.4 9.2 6 20.6 11.4 8.1 15 25 15

Additional Resources and Comments