Overview Data source: UniProt

Official Gene Symbol Other Aliases
EPCAM GA733-2,M1S2,M4S1,MIC18,TACSTD1,TROP1
Sequence Length (AA) Molecular Weight (Da)
314 34932
Protein Name
Epithelial cell adhesion molecule
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPPQVLAFG LLLAAATATF AAAQEECVCE NYKLAVNCFV NNNRQCQCTS
60 70 80 90 100
VGAQNTVICS KLAAKCLVMK AEMNGSKLGR RAKPEGALQN NDGLYDPDCD
110 120 130 140 150
ESGLFKAKQC NGTSMCWCVN TAGVRRTDKD TEITCSERVR TYWIIIELKH
160 170 180 190 200
KAREKPYDSK SLRTALQKEI TTRYQLDPKF ITSILYENNV ITIDLVQNSS
210 220 230 240 250
QKTQNDVDIA DVAYYFEKDV KGESLFHSKK MDLTVNGEQL DLDPGQTLIY
260 270 280 290 300
YVDEKAPEFS MQGLKAGVIA VIVVVVIAVV AGIVVLVISR KKRMAKYEKA
310 314
EIKEMGEMHR ELNA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-503 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPCAM
Peptide Modified Sequence
AKPEGALQNNDGLYDPDC[+57.0]DESGLFK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
99
Peptide - Site of Modification
18
Peptide Start
82
Peptide End
106
CPTAC ID
CPTAC-503
Peptide Molecular Mass
2,752.2286
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 21.1 7.7 13.7 28.5 14.3 20.3 35.5 16.2 24.5 15 25 15
y9 (1+) 50.6 22.6 33.1 58.2 21.3 44.8 77.1 31.1 55.7 15 25 15
y8 (1+) 35.6 23 14.1 37 26.2 28.6 51.3 34.9 31.9 15 25 15
sum 22.6 8.4 11.5 28.6 14 20.2 36.5 16.3 23.2 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-502 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPCAM
Peptide Modified Sequence
LAVNC[+57.0]FVNNNR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
38
Peptide - Site of Modification
5
Peptide Start
34
Peptide End
44
CPTAC ID
CPTAC-502
Peptide Molecular Mass
1,319.6405
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 29 9.7 11.6 44.7 9.6 14.6 53.3 13.6 18.6 15 25 15
y7 (1+) 29.5 10.4 11.6 43.7 10.3 18.4 52.7 14.6 21.8 15 25 15
y6 (1+) 28.1 11.9 8 69.1 22.1 10.1 74.6 25.1 12.9 15 25 15
sum 27.6 10.3 8.7 68 15 12.6 73.4 18.2 15.3 15 25 15

Additional Resources and Comments