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Overview Data source: UniProt

Official Gene Symbol Other Aliases
LANCL2 GPR69B,TASP
Sequence Length (AA) Molecular Weight (Da)
450 50854
Protein Name
LanC-like protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGETMSKRLK LHLGGEAEME ERAFVNPFPD YEAAAGALLA SGAAEETGCV
60 70 80 90 100
RPPATTDEPG LPFHQDGKII HNFIRRIQTK IKDLLQQMEE GLKTADPHDC
110 120 130 140 150
SAYTGWTGIA LLYLQLYRVT CDQTYLLRSL DYVKRTLRNL NGRRVTFLCG
160 170 180 190 200
DAGPLAVGAV IYHKLRSDCE SQECVTKLLQ LQRSVVCQES DLPDELLYGR
210 220 230 240 250
AGYLYALLYL NTEIGPGTVC ESAIKEVVNA IIESGKTLSR EERKTERCPL
260 270 280 290 300
LYQWHRKQYV GAAHGMAGIY YMLMQPAAKV DQETLTEMVK PSIDYVRHKK
310 320 330 340 350
FRSGNYPSSL SNETDRLVHW CHGAPGVIHM LMQAYKVFKE EKYLKEAMEC
360 370 380 390 400
SDVIWQRGLL RKGYGICHGT AGNGYSFLSL YRLTQDKKYL YRACKFAEWC
410 420 430 440 450
LDYGAHGCRI PDRPYSLFEG MAGAIHFLSD VLGPETSRFP AFELDSSKRD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-533 Collapse assay details

Data source: Panorama

Official Gene Symbol
LANCL2
Peptide Sequence
FPAFELDSSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
439
Peptide End
448
CPTAC ID
CPTAC-533
Peptide Molecular Mass
1,139.5499
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 18.4 8.9 8.6 31.9 20.3 20.4 36.8 22.2 22.1 15 15 15
y7 (1+) 15.2 5.7 3.5 15.2 6.6 7.3 21.5 8.7 8.1 15 15 15
y6 (1+) 30.5 7.6 9.7 39.9 6.4 11.2 50.2 9.9 14.8 15 15 15
sum 14.5 5.1 4.8 22.2 10.1 8.2 26.5 11.3 9.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-532 Collapse assay details

Data source: Panorama

Official Gene Symbol
LANCL2
Peptide Sequence
SGNYPSSLSNETDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
303
Peptide End
316
CPTAC ID
CPTAC-532
Peptide Molecular Mass
1,525.6645
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 12.7 6.7 10.4 19.1 12 13.6 22.9 13.7 17.1 15 25 15
y9 (1+) 16.2 14.5 20.6 22.9 21.2 22.2 28.1 25.7 30.3 15 25 15
y8 (1+) 26.7 19.3 11.4 28.1 24.5 26.5 38.8 31.2 28.8 15 25 15
sum 12.1 7.5 10.5 18.5 13.1 10.9 22.1 15.1 15.1 15 25 15

Additional Resources and Comments