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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MARS N/A
Sequence Length (AA) Molecular Weight (Da)
900 101116
Protein Name
Methionine--tRNA ligase, cytoplasmic
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLFVSDGVP GCLPVLAAAG RARGRAEVLI STVGPEDCVV PFLTRPKVPV
60 70 80 90 100
LQLDSGNYLF STSAICRYFF LLSGWEQDDL TNQWLEWEAT ELQPALSAAL
110 120 130 140 150
YYLVVQGKKG EDVLGSVRRA LTHIDHSLSR QNCPFLAGET ESLADIVLWG
160 170 180 190 200
ALYPLLQDPA YLPEELSALH SWFQTLSTQE PCQRAAETVL KQQGVLALRP
210 220 230 240 250
YLQKQPQPSP AEGRAVTNEP EEEELATLSE EEIAMAVTAW EKGLESLPPL
260 270 280 290 300
RPQQNPVLPV AGERNVLITS ALPYVNNVPH LGNIIGCVLS ADVFARYSRL
310 320 330 340 350
RQWNTLYLCG TDEYGTATET KALEEGLTPQ EICDKYHIIH ADIYRWFNIS
360 370 380 390 400
FDIFGRTTTP QQTKITQDIF QQLLKRGFVL QDTVEQLRCE HCARFLADRF
410 420 430 440 450
VEGVCPFCGY EEARGDQCDK CGKLINAVEL KKPQCKVCRS CPVVQSSQHL
460 470 480 490 500
FLDLPKLEKR LEEWLGRTLP GSDWTPNAQF ITRSWLRDGL KPRCITRDLK
510 520 530 540 550
WGTPVPLEGF EDKVFYVWFD ATIGYLSITA NYTDQWERWW KNPEQVDLYQ
560 570 580 590 600
FMAKDNVPFH SLVFPCSALG AEDNYTLVSH LIATEYLNYE DGKFSKSRGV
610 620 630 640 650
GVFGDMAQDT GIPADIWRFY LLYIRPEGQD SAFSWTDLLL KNNSELLNNL
660 670 680 690 700
GNFINRAGMF VSKFFGGYVP EMVLTPDDQR LLAHVTLELQ HYHQLLEKVR
710 720 730 740 750
IRDALRSILT ISRHGNQYIQ VNEPWKRIKG SEADRQRAGT VTGLAVNIAA
760 770 780 790 800
LLSVMLQPYM PTVSATIQAQ LQLPPPACSI LLTNFLCTLP AGHQIGTVSP
810 820 830 840 850
LFQKLENDQI ESLRQRFGGG QAKTSPKPAV VETVTTAKPQ QIQALMDEVT
860 870 880 890 900
KQGNIVRELK AQKADKNEVA AEVAKLLDLK KQLAVAEGKP PEAPKGKKKK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-538 Collapse assay details

Data source: Panorama

Official Gene Symbol
MARS
Peptide Modified Sequence
AEVLISTVGPEDC[+57.0]VVPFLTRPK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
38
Peptide - Site of Modification
13
Peptide Start
26
Peptide End
47
CPTAC ID
CPTAC-538
Peptide Molecular Mass
2,426.2879
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 31.6 27 37 40.5 38.9 44.8 51.4 47.4 58.1 15 15 15
y18 (2+) 19.1 24.6 16.6 23.8 25.2 19.5 30.5 35.2 25.6 15 15 15
y17 (2+) 25.8 13 12.6 31.5 18.3 23 40.7 22.4 26.2 15 15 15
sum 20.7 14.7 10.3 23.8 19.4 19.7 31.5 24.3 22.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-539 Collapse assay details

Data source: Panorama

Official Gene Symbol
MARS
Peptide Sequence
GFVLQDTVEQLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
377
Peptide End
388
CPTAC ID
CPTAC-539
Peptide Molecular Mass
1,403.7409
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 13.7 8.8 6.7 16 11.5 6.9 21.1 14.5 9.6 15 25 15
y8 (1+) 16.5 6.7 5.9 24.3 9.4 6.1 29.4 11.5 8.5 15 25 15
y6 (1+) 21.8 13.1 18.9 24.8 15.3 20.5 33 20.1 27.9 15 25 15
sum 7.5 6.9 7 13.3 9.4 7.4 15.3 11.7 10.2 15 25 15

Additional Resources and Comments