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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MBOAT7 BB1, LENG4, OACT7
Sequence Length (AA) Molecular Weight (Da)
472 52765
Protein Name
Lysophospholipid acyltransferase 7
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSPEEWTYLV VLLISIPIGF LFKKAGPGLK RWGAAAVGLG LTLFTCGPHT
60 70 80 90 100
LHSLVTILGT WALIQAQPCS CHALALAWTF SYLLFFRALS LLGLPTPTPF
110 120 130 140 150
TNAVQLLLTL KLVSLASEVQ DLHLAQRKEM ASGFSKGPTL GLLPDVPSLM
160 170 180 190 200
ETLSYSYCYV GIMTGPFFRY RTYLDWLEQP FPGAVPSLRP LLRRAWPAPL
210 220 230 240 250
FGLLFLLSSH LFPLEAVRED AFYARPLPAR LFYMIPVFFA FRMRFYVAWI
260 270 280 290 300
AAECGCIAAG FGAYPVAAKA RAGGGPTLQC PPPSSPEKAA SLEYDYETIR
310 320 330 340 350
NIDCYSTDFC VRVRDGMRYW NMTVQWWLAQ YIYKSAPARS YVLRSAWTML
360 370 380 390 400
LSAYWHGLHP GYYLSFLTIP LCLAAEGRLE SALRGRLSPG GQKAWDWVHW
410 420 430 440 450
FLKMRAYDYM CMGFVLLSLA DTLRYWASIY FCIHFLALAA LGLGLALGGG
460 470 472
SPSRRKAASQ PTSLAPEKLR EE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-541 Collapse assay details

Data source: Panorama

Official Gene Symbol
MBOAT7
Peptide Sequence
AASLEYDYETIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
289
Peptide End
300
CPTAC ID
CPTAC-541
Peptide Molecular Mass
1,429.6725
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 17.1 7.4 7.4 24.1 8.4 8.7 29.6 11.2 11.4 15 25 15
y7 (1+) 16.1 9.8 4.2 20.4 10.1 6.9 26 14.1 8.1 15 25 15
y6 (1+) 26.7 9.7 8.3 23.2 8.6 8.6 35.4 13 12 15 25 15
sum 10.2 7.4 3.1 15.6 7.8 5.7 18.6 10.8 6.5 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-540 Collapse assay details

Data source: Panorama

Official Gene Symbol
MBOAT7
Peptide Modified Sequence
AGGGPTLQC[+57.0]PPPSSPEK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
280
Peptide - Site of Modification
9
Peptide Start
272
Peptide End
288
CPTAC ID
CPTAC-540
Peptide Molecular Mass
1,678.7985
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 22.5 9.3 9.6 24 9.9 11 32.9 13.6 14.6 15 25 15
y9 (1+) 23.5 9.1 8.2 26.5 8.9 9.5 35.4 12.7 12.5 15 25 15
y13 (2+) 32 9.7 15.6 46.1 9.4 16 56.1 13.5 22.3 15 25 15
sum 18.7 7.9 9.2 28.2 7.7 10.2 33.8 11 13.7 15 25 15


Additional Resources and Comments