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Overview Data source: UniProt

Official Gene Symbol Other Aliases
NIT2 N/A
Sequence Length (AA) Molecular Weight (Da)
276 30608
Protein Name
Omega-amidase NIT2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTSFRLALIQ LQISSIKSDN VTRACSFIRE AATQGAKIVS LPECFNSPYG
60 70 80 90 100
AKYFPEYAEK IPGESTQKLS EVAKECSIYL IGGSIPEEDA GKLYNTCAVF
110 120 130 140 150
GPDGTLLAKY RKIHLFDIDV PGKITFQESK TLSPGDSFST FDTPYCRVGL
160 170 180 190 200
GICYDMRFAE LAQIYAQRGC QLLVYPGAFN LTTGPAHWEL LQRSRAVDNQ
210 220 230 240 250
VYVATASPAR DDKASYVAWG HSTVVNPWGE VLAKAGTEEA IVYSDIDLKK
260 270 276
LAEIRQQIPV FRQKRSDLYA VEMKKP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-549 Collapse assay details

Data source: Panorama

Official Gene Symbol
NIT2
Peptide Modified Sequence
EC[+57.0]SIYLIGGSIPEEDAGK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
76
Peptide - Site of Modification
2
Peptide Start
75
Peptide End
92
CPTAC ID
CPTAC-549
Peptide Molecular Mass
1,936.9088
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 45.9 16.3 16.3 63.2 23.8 19.1 78.1 28.8 25.1 15 25 15
y11 (1+) 36.1 9.6 23.8 57.2 16.6 30.4 67.6 19.2 38.6 15 25 15
y10 (1+) 54.1 21.1 31.6 64.5 23.4 31.4 84.2 31.5 44.5 15 25 15
sum 39 10.5 20.1 59.3 17.8 22.4 71 20.7 30.1 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-550 Collapse assay details

Data source: Panorama

Official Gene Symbol
NIT2
Peptide Modified Sequence
TLSPGDSFSTFDTPYC[+57.0]R
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
146
Peptide - Site of Modification
16
Peptide Start
131
Peptide End
147
CPTAC ID
CPTAC-550
Peptide Molecular Mass
1,949.8465
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 17.5 8.6 12.8 22.7 11.9 11.8 28.7 14.7 17.4 15 25 15
y15 (2+) 15 9.8 5.1 18.1 10.1 8.8 23.5 14.1 10.2 15 25 15
y14 (2+) 9.3 7.5 6 13.1 8.8 7.7 16.1 11.6 9.8 15 25 15
sum 8.3 6.9 5.1 12.6 8.9 4.5 15.1 11.3 6.8 15 25 15

Additional Resources and Comments