Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

NUP155

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
NUP155 KIAA0791
Sequence Length (AA) Molecular Weight (Da)
1391 155199
Protein Name
Nuclear pore complex protein Nup155
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPSSLLGAAM PASTSAAALQ EALENAGRLI DRQLQEDRMY PDLSELLMVS
60 70 80 90 100
APNNPTVSGM SDMDYPLQGP GLLSVPNLPE ISSIRRVPLP PELVEQFGHM
110 120 130 140 150
QCNCMMGVFP PISRAWLTID SDIFMWNYED GGDLAYFDGL SETILAVGLV
160 170 180 190 200
KPKAGIFQPH VRHLLVLATP VDIVILGLSY ANLQTGSGVL NDSLSGGMQL
210 220 230 240 250
LPDPLYSLPT DNTYLLTITS TDNGRIFLAG KDGCLYEVAY QAEAGWFSQR
260 270 280 290 300
CRKINHSKSS LSFLVPSLLQ FTFSEDDPIL QIAIDNSRNI LYTRSEKGVI
310 320 330 340 350
QVYDLGQDGQ GMSRVASVSQ NAIVSAAGNI ARTIDRSVFK PIVQIAVIEN
360 370 380 390 400
SESLDCQLLA VTHAGVRLYF STCPFRQPLA RPNTLTLVHV RLPPGFSASS
410 420 430 440 450
TVEKPSKVHR ALYSKGILLM AASENEDNDI LWCVNHDTFP FQKPMMETQM
460 470 480 490 500
TAGVDGHSWA LSAIDELKVD KIITPLNKDH IPITDSPVVV QQHMLPPKKF
510 520 530 540 550
VLLSAQGSLM FHKLRPVDQL RHLLVSNVGG DGEEIERFFK LHQEDQACAT
560 570 580 590 600
CLILACSTAA CDREVSAWAT RAFFRYGGEA QMRFPTTLPP PSNVGPILGS
610 620 630 640 650
PVYSSSPVPS GSPYPNPSFL GTPSHGIQPP AMSTPVCALG NPATQATNMS
660 670 680 690 700
CVTGPEIVYS GKHNGICIYF SRIMGNIWDA SLVVERIFKS GNREITAIES
710 720 730 740 750
SVPCQLLESV LQELKGLQEF LDRNSQFAGG PLGNPNTTAK VQQRLIGFMR
760 770 780 790 800
PENGNPQQMQ QELQRKFHEA QLSEKISLQA IQQLVRKSYQ ALALWKLLCE
810 820 830 840 850
HQFTIIVAEL QKELQEQLKI TTFKDLVIRD KELTGALIAS LINCYIRDNA
860 870 880 890 900
AVDGISLHLQ DICPLLYSTD DAICSKANEL LQRSRQVQNK TEKERMLRES
910 920 930 940 950
LKEYQKISNQ VDLSNVCAQY RQVRFYEGVV ELSLTAAEKK DPQGLGLHFY
960 970 980 990 1000
KHGEPEEDIV GLQAFQERLN SYKCITDTLQ ELVNQSKAAP QSPSVPKKPG
1010 1020 1030 1040 1050
PPVLSSDPNM LSNEEAGHHF EQMLKLSQRS KDELFSIALY NWLIQVDLAD
1060 1070 1080 1090 1100
KLLQVASPFL EPHLVRMAKV DQNRVRYMDL LWRYYEKNRS FSNAARVLSR
1110 1120 1130 1140 1150
LADMHSTEIS LQQRLEYIAR AILSAKSSTA ISSIAADGEF LHELEEKMEV
1160 1170 1180 1190 1200
ARIQLQIQET LQRQYSHHSS VQDAVSQLDS ELMDITKLYG EFADPFKLAE
1210 1220 1230 1240 1250
CKLAIIHCAG YSDPILVQTL WQDIIEKELS DSVTLSSSDR MHALSLKIVL
1260 1270 1280 1290 1300
LGKIYAGTPR FFPLDFIVQF LEQQVCTLNW DVGFVIQTMN EIGVPLPRLL
1310 1320 1330 1340 1350
EVYDQLFKSR DPFWNRMKKP LHLLDCIHVL LIRYVENPSQ VLNCERRRFT
1360 1370 1380 1390 1391
NLCLDAVCGY LVELQSMSSS VAVQAITGNF KSLQAKLERL H

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-553 Collapse assay details

Data source: Panorama

Official Gene Symbol
NUP155
Peptide Sequence
VASVSQNAIVSAAGNIAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
315
Peptide End
332
CPTAC ID
CPTAC-553
Peptide Molecular Mass
1,726.9326
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 20.1 13.6 13.5 24.9 13.8 11.8 32 19.4 17.9 15 25 15
y10 (1+) 17.2 14.9 12.8 17.1 17.5 13.8 24.3 23 18.8 15 25 15
y8 (1+) 12.2 10.4 11 18.1 14.8 11.8 21.8 18.1 16.1 15 25 15
sum 6.4 9.3 11.4 14.8 12.7 10.1 16.1 15.7 15.2 15 25 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments