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Overview Data source: UniProt

Official Gene Symbol Other Aliases
PLOD2 N/A
Sequence Length (AA) Molecular Weight (Da)
737 84686
Protein Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGGCTVKPQL LLLALVLHPW NPCLGADSEK PSSIPTDKLL VITVATKESD
60 70 80 90 100
GFHRFMQSAK YFNYTVKVLG QGEEWRGGDG INSIGGGQKV RLMKEVMEHY
110 120 130 140 150
ADQDDLVVMF TECFDVIFAG GPEEVLKKFQ KANHKVVFAA DGILWPDKRL
160 170 180 190 200
ADKYPVVHIG KRYLNSGGFI GYAPYVNRIV QQWNLQDNDD DQLFYTKVYI
210 220 230 240 250
DPLKREAINI TLDHKCKIFQ TLNGAVDEVV LKFENGKARA KNTFYETLPV
260 270 280 290 300
AINGNGPTKI LLNYFGNYVP NSWTQDNGCT LCEFDTVDLS AVDVHPNVSI
310 320 330 340 350
GVFIEQPTPF LPRFLDILLT LDYPKEALKL FIHNKEVYHE KDIKVFFDKA
360 370 380 390 400
KHEIKTIKIV GPEENLSQAE ARNMGMDFCR QDEKCDYYFS VDADVVLTNP
410 420 430 440 450
RTLKILIEQN RKIIAPLVTR HGKLWSNFWG ALSPDGYYAR SEDYVDIVQG
460 470 480 490 500
NRVGVWNVPY MANVYLIKGK TLRSEMNERN YFVRDKLDPD MALCRNAREM
510 520 530 540 550
GVFMYISNRH EFGRLLSTAN YNTSHYNNDL WQIFENPVDW KEKYINRDYS
560 570 580 590 600
KIFTENIVEQ PCPDVFWFPI FSEKACDELV EEMEHYGKWS GGKHHDSRIS
610 620 630 640 650
GGYENVPTDD IHMKQVDLEN VWLHFIREFI APVTLKVFAG YYTKGFALLN
660 670 680 690 700
FVVKYSPERQ RSLRPHHDAS TFTINIALNN VGEDFQGGGC KFLRYNCSIE
710 720 730 737
SPRKGWSFMH PGRLTHLHEG LPVKNGTRYI AVSFIDP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-568 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLOD2
Peptide Sequence
IVGPEENLSQAEAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
359
Peptide End
372
CPTAC ID
CPTAC-568
Peptide Molecular Mass
1,511.7580
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 18.1 3.6 5.7 24.1 4.8 9.2 30.1 6 10.8 15 15 15
y8 (1+) 16.9 7.7 7.1 19.8 8 7.8 26 11.1 10.5 15 15 15
y7 (1+) 16.2 7.1 7.1 14.2 8.7 7.8 21.5 11.2 10.5 15 15 15
sum 7.9 3.4 3.6 11.4 4 4.6 13.9 5.2 5.8 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-569 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLOD2
Peptide Sequence
SEDYVDIVQGNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
441
Peptide End
452
CPTAC ID
CPTAC-569
Peptide Molecular Mass
1,393.6474
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 15 17.4 17.9 20.5 17.3 33.4 25.4 24.5 37.9 15 25 15
y8 (1+) 23.2 22.6 20.6 24 21.9 20.3 33.4 31.5 28.9 15 25 15
y7 (1+) 9.8 12.9 12.2 15.4 10.9 12.3 18.3 16.9 17.3 15 25 15
sum 9.2 11.1 7.9 12 9.1 13 15.1 14.4 15.2 15 25 15

Additional Resources and Comments