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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
DNM1L DLP1, DRP1
Sequence Length (AA) Molecular Weight (Da)
736 81877
Protein Name
Dynamin-1-like protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEALIPVINK LQDVFNTVGA DIIQLPQIVV VGTQSSGKSS VLESLVGRDL
60 70 80 90 100
LPRGTGIVTR RPLILQLVHV SQEDKRKTTG EENGVEAEEW GKFLHTKNKL
110 120 130 140 150
YTDFDEIRQE IENETERISG NNKGVSPEPI HLKIFSPNVV NLTLVDLPGM
160 170 180 190 200
TKVPVGDQPK DIELQIRELI LRFISNPNSI ILAVTAANTD MATSEALKIS
210 220 230 240 250
REVDPDGRRT LAVITKLDLM DAGTDAMDVL MGRVIPVKLG IIGVVNRSQL
260 270 280 290 300
DINNKKSVTD SIRDEYAFLQ KKYPSLANRN GTKYLARTLN RLLMHHIRDC
310 320 330 340 350
LPELKTRINV LAAQYQSLLN SYGEPVDDKS ATLLQLITKF ATEYCNTIEG
360 370 380 390 400
TAKYIETSEL CGGARICYIF HETFGRTLES VDPLGGLNTI DILTAIRNAT
410 420 430 440 450
GPRPALFVPE VSFELLVKRQ IKRLEEPSLR CVELVHEEMQ RIIQHCSNYS
460 470 480 490 500
TQELLRFPKL HDAIVEVVTC LLRKRLPVTN EMVHNLVAIE LAYINTKHPD
510 520 530 540 550
FADACGLMNN NIEEQRRNRL ARELPSAVSR DKSSKVPSAL APASQEPSPA
560 570 580 590 600
ASAEADGKLI QDSRRETKNV ASGGGGVGDG VQEPTTGNWR GMLKTSKAEE
610 620 630 640 650
LLAEEKSKPI PIMPASPQKG HAVNLLDVPV PVARKLSARE QRDCEVIERL
660 670 680 690 700
IKSYFLIVRK NIQDSVPKAV MHFLVNHVKD TLQSELVGQL YKSSLLDDLL
710 720 730 736
TESEDMAQRR KEAADMLKAL QGASQIIAEI RETHLW

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-57 Collapse assay details

Data source: Panorama

Official Gene Symbol
DNM1L
Peptide Sequence
DTLQSELVGQLYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
680
Peptide End
692
CPTAC ID
CPTAC-57
Peptide Molecular Mass
1,492.7773
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 12.9 10.9 8.2 19.1 18.8 9.9 23 21.7 12.9 15 25 15
y8 (1+) 18.6 23.2 19.6 21.7 25.7 27.2 28.6 34.6 33.5 15 25 15
y7 (1+) 9.7 15.1 12.5 12.4 25.7 16.3 15.7 29.8 20.5 15 25 15
sum 10 10 6.1 14.3 20.3 11 17.4 22.6 12.6 15 25 15

Selectivity

Data source: Panorama

Slope of Curve Fit for cell line
fragment_ion HCC1187 HCC1419 HCC1954 HCC70 MCF12A MDAMB175VII ZR751 ZR7530
y7 (1+) -1.93 -9.62 -7.96 -6.28 -3.09 -5.69 -2.84 -6.15
y9 (1+) -3.28 -8.16 -14.62 -9.17 -3.31 -7.99 -3.03 -7.9
y8 (1+) -2.72 -7.23 -10.94 -8.97 -2.88 -7.16 -2.41 -11.27
sum -2.67 -8.38 -11.28 -8.12 -3.16 -7.09 -2.88 -7.61

Stability

Data source: Panorama

Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y9 (1+) 16.4 14.7 11 NA 7.5
y8 (1+) 29.6 19.2 11.5 NA 11.7
y7 (1+) 19.6 9.8 3.8 NA 32
sum 17 13.6 6.5 NA 13.2
Fragment ion / Transition all_intra_CV all_inter_CV
y9 (1+) 12.9 11.3
y8 (1+) 18.2 17.2
y7 (1+) 15 17.1
sum 12.8 11.4

Endogenous

Data source: Panorama

Fragment ion / Transition intra_CV inter_CV total_CV
y8 (1+) 22.6 28.7 36.5
y9 (1+) 11.3 20.8 23.7
y7 (1+) 14.7 30.1 33.5
sum 10.1 23.3 25.4

Additional Resources and Comments