DNM1L, CPTAC-57 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DNM1L	DLP1, DRP1

Sequence Length (AA)	Molecular Weight (Da)
736	81877

Protein Name
Dynamin-1-like protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEALIPVINK	LQDVFNTVGA	DIIQLPQIVV	VGTQSSGKSS	VLESLVGRDL
60	70	80	90	100
LPRGTGIVTR	RPLILQLVHV	SQEDKRKTTG	EENGVEAEEW	GKFLHTKNKL
110	120	130	140	150
YTDFDEIRQE	IENETERISG	NNKGVSPEPI	HLKIFSPNVV	NLTLVDLPGM
160	170	180	190	200
TKVPVGDQPK	DIELQIRELI	LRFISNPNSI	ILAVTAANTD	MATSEALKIS
210	220	230	240	250
REVDPDGRRT	LAVITKLDLM	DAGTDAMDVL	MGRVIPVKLG	IIGVVNRSQL
260	270	280	290	300
DINNKKSVTD	SIRDEYAFLQ	KKYPSLANRN	GTKYLARTLN	RLLMHHIRDC
310	320	330	340	350
LPELKTRINV	LAAQYQSLLN	SYGEPVDDKS	ATLLQLITKF	ATEYCNTIEG
360	370	380	390	400
TAKYIETSEL	CGGARICYIF	HETFGRTLES	VDPLGGLNTI	DILTAIRNAT
410	420	430	440	450
GPRPALFVPE	VSFELLVKRQ	IKRLEEPSLR	CVELVHEEMQ	RIIQHCSNYS
460	470	480	490	500
TQELLRFPKL	HDAIVEVVTC	LLRKRLPVTN	EMVHNLVAIE	LAYINTKHPD
510	520	530	540	550
FADACGLMNN	NIEEQRRNRL	ARELPSAVSR	DKSSKVPSAL	APASQEPSPA
560	570	580	590	600
ASAEADGKLI	QDSRRETKNV	ASGGGGVGDG	VQEPTTGNWR	GMLKTSKAEE
610	620	630	640	650
LLAEEKSKPI	PIMPASPQKG	HAVNLLDVPV	PVARKLSARE	QRDCEVIERL
660	670	680	690	700
IKSYFLIVRK	NIQDSVPKAV	MHFLVNHVKD	TLQSELVGQL	YKSSLLDDLL
710	720	730	736
TESEDMAQRR	KEAADMLKAL	QGASQIIAEI	RETHLW

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-57 Collapse assay details

Data source: Panorama

Official Gene Symbol: DNM1L
Peptide Sequence: DTLQSELVGQLYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 680
Peptide End: 692

CPTAC ID: CPTAC-57
Peptide Molecular Mass: 1,492.7773
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	12.9	10.9	8.2	19.1	18.8	9.9	23	21.7	12.9	15	25	15
y8 (1+)	18.6	23.2	19.6	21.7	25.7	27.2	28.6	34.6	33.5	15	25	15
y7 (1+)	9.7	15.1	12.5	12.4	25.7	16.3	15.7	29.8	20.5	15	25	15
sum	10	10	6.1	14.3	20.3	11	17.4	22.6	12.6	15	25	15

Selectivity

Data source: Panorama

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	Slope of Curve Fit for cell line
fragment_ion	HCC1187	HCC1419	HCC1954	HCC70	MCF12A	MDAMB175VII	ZR751	ZR7530
y7 (1+)	-1.93	-9.62	-7.96	-6.28	-3.09	-5.69	-2.84	-6.15
y9 (1+)	-3.28	-8.16	-14.62	-9.17	-3.31	-7.99	-3.03	-7.9
y8 (1+)	-2.72	-7.23	-10.94	-8.97	-2.88	-7.16	-2.41	-11.27
sum	-2.67	-8.38	-11.28	-8.12	-3.16	-7.09	-2.88	-7.61

Stability

Data source: Panorama

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Fragment ion / Transition	control_intra_CV	actual_temp_intra_CV	frozen_intra_CV	FTx1_intra_CV	FTx2_intra_CV
y9 (1+)	16.4	14.7	11	NA	7.5
y8 (1+)	29.6	19.2	11.5	NA	11.7
y7 (1+)	19.6	9.8	3.8	NA	32
sum	17	13.6	6.5	NA	13.2

Fragment ion / Transition	all_intra_CV	all_inter_CV
y9 (1+)	12.9	11.3
y8 (1+)	18.2	17.2
y7 (1+)	15	17.1
sum	12.8	11.4

Endogenous

Data source: Panorama

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Fragment ion / Transition	intra_CV	inter_CV	total_CV
y8 (1+)	22.6	28.7	36.5
y9 (1+)	11.3	20.8	23.7
y7 (1+)	14.7	30.1	33.5
sum	10.1	23.3	25.4

DNM1L

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-57 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Selectivity

Stability

Endogenous

Additional Resources and Comments

Comments

Search form

DNM1L

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-57 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Selectivity

Stability

Endogenous

Additional Resources and Comments

Comments