DPP3, CPTAC-58 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DPP3	N/A

Sequence Length (AA)	Molecular Weight (Da)
737	82589

Protein Name
Dipeptidyl peptidase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MADTQYILPN	DIGVSSLDCR	EAFRLLSPTE	RLYAYHLSRA	AWYGGLAVLL
60	70	80	90	100
QTSPEAPYIY	ALLSRLFRAQ	DPDQLRQHAL	AEGLTEEEYQ	AFLVYAAGVY
110	120	130	140	150
SNMGNYKSFG	DTKFVPNLPK	EKLERVILGS	EAAQQHPEEV	RGLWQTCGEL
160	170	180	190	200
MFSLEPRLRH	LGLGKEGITT	YFSGNCTMED	AKLAQDFLDS	QNLSAYNTRL
210	220	230	240	250
FKEVDGEGKP	YYEVRLASVL	GSEPSLDSEV	TSKLKSYEFR	GSPFQVTRGD
260	270	280	290	300
YAPILQKVVE	QLEKAKAYAA	NSHQGQMLAQ	YIESFTQGSI	EAHKRGSRFW
310	320	330	340	350
IQDKGPIVES	YIGFIESYRD	PFGSRGEFEG	FVAVVNKAMS	AKFERLVASA
360	370	380	390	400
EQLLKELPWP	PTFEKDKFLT	PDFTSLDVLT	FAGSGIPAGI	NIPNYDDLRQ
410	420	430	440	450
TEGFKNVSLG	NVLAVAYATQ	REKLTFLEED	DKDLYILWKG	PSFDVQVGLH
460	470	480	490	500
ELLGHGSGKL	FVQDEKGAFN	FDQETVINPE	TGEQIQSWYR	SGETWDSKFS
510	520	530	540	550
TIASSYEECR	AESVGLYLCL	HPQVLEIFGF	EGADAEDVIY	VNWLNMVRAG
560	570	580	590	600
LLALEFYTPE	AFNWRQAHMQ	ARFVILRVLL	EAGEGLVTIT	PTTGSDGRPD
610	620	630	640	650
ARVRLDRSKI	RSVGKPALER	FLRRLQVLKS	TGDVAGGRAL	YEGYATVTDA
660	670	680	690	700
PPECFLTLRD	TVLLRKESRK	LIVQPNTRLE	GSDVQLLEYE	ASAAGLIRSF
710	720	730	737
SERFPEDGPE	LEEILTQLAT	ADARFWKGPS	EAPSGQA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-58 Collapse assay details

Data source: Panorama

Official Gene Symbol: DPP3
Peptide Sequence: LASVLGSEPSLDSEVTSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 216
Peptide End: 233

CPTAC ID: CPTAC-58
Peptide Molecular Mass: 1,817.9258
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	25.3	9.7	6.1	31.9	9.8	12.6	40.7	13.8	14	15	25	15
y10 (1+)	10.3	5.2	5	14.4	5.9	9.7	17.7	7.9	10.9	15	25	15
y14 (2+)	41	14.9	15.8	41.6	14.5	15.2	58.4	20.8	21.9	15	25	15
sum	9.3	4.1	3.8	11.7	4.7	7.4	14.9	6.2	8.3	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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