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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
DPP3 N/A
Sequence Length (AA) Molecular Weight (Da)
737 82589
Protein Name
Dipeptidyl peptidase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MADTQYILPN DIGVSSLDCR EAFRLLSPTE RLYAYHLSRA AWYGGLAVLL
60 70 80 90 100
QTSPEAPYIY ALLSRLFRAQ DPDQLRQHAL AEGLTEEEYQ AFLVYAAGVY
110 120 130 140 150
SNMGNYKSFG DTKFVPNLPK EKLERVILGS EAAQQHPEEV RGLWQTCGEL
160 170 180 190 200
MFSLEPRLRH LGLGKEGITT YFSGNCTMED AKLAQDFLDS QNLSAYNTRL
210 220 230 240 250
FKEVDGEGKP YYEVRLASVL GSEPSLDSEV TSKLKSYEFR GSPFQVTRGD
260 270 280 290 300
YAPILQKVVE QLEKAKAYAA NSHQGQMLAQ YIESFTQGSI EAHKRGSRFW
310 320 330 340 350
IQDKGPIVES YIGFIESYRD PFGSRGEFEG FVAVVNKAMS AKFERLVASA
360 370 380 390 400
EQLLKELPWP PTFEKDKFLT PDFTSLDVLT FAGSGIPAGI NIPNYDDLRQ
410 420 430 440 450
TEGFKNVSLG NVLAVAYATQ REKLTFLEED DKDLYILWKG PSFDVQVGLH
460 470 480 490 500
ELLGHGSGKL FVQDEKGAFN FDQETVINPE TGEQIQSWYR SGETWDSKFS
510 520 530 540 550
TIASSYEECR AESVGLYLCL HPQVLEIFGF EGADAEDVIY VNWLNMVRAG
560 570 580 590 600
LLALEFYTPE AFNWRQAHMQ ARFVILRVLL EAGEGLVTIT PTTGSDGRPD
610 620 630 640 650
ARVRLDRSKI RSVGKPALER FLRRLQVLKS TGDVAGGRAL YEGYATVTDA
660 670 680 690 700
PPECFLTLRD TVLLRKESRK LIVQPNTRLE GSDVQLLEYE ASAAGLIRSF
710 720 730 737
SERFPEDGPE LEEILTQLAT ADARFWKGPS EAPSGQA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-58 Collapse assay details

Data source: Panorama

Official Gene Symbol
DPP3
Peptide Sequence
LASVLGSEPSLDSEVTSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
216
Peptide End
233
CPTAC ID
CPTAC-58
Peptide Molecular Mass
1,817.9258
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 25.3 9.7 6.1 31.9 9.8 12.6 40.7 13.8 14 15 25 15
y10 (1+) 10.3 5.2 5 14.4 5.9 9.7 17.7 7.9 10.9 15 25 15
y14 (2+) 41 14.9 15.8 41.6 14.5 15.2 58.4 20.8 21.9 15 25 15
sum 9.3 4.1 3.8 11.7 4.7 7.4 14.9 6.2 8.3 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-59 Collapse assay details

Data source: Panorama

Official Gene Symbol
DPP3
Peptide Sequence
VLLEAGEGLVTITPTTGSDGRPDAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
578
Peptide End
602
CPTAC ID
CPTAC-59
Peptide Molecular Mass
2,524.3133
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y22 (2+) 7.5 11.4 13.4 13.2 16.2 32.1 15.2 19.8 34.8 25 15 15
y20 (2+) 7.1 6.4 7.9 12.7 10.8 11.5 14.5 12.6 14 25 15 15
y18 (2+) 8.9 6.7 10.5 13.9 16.6 18.4 16.5 17.9 21.2 25 15 15
sum 5.6 5.8 6.2 12.1 13 15.6 13.3 14.2 16.8 25 15 15


Additional Resources and Comments