SPTBN1, CPTAC-590 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SPTBN1	SPTB2

Sequence Length (AA)	Molecular Weight (Da)
2364	274609

Protein Name
Spectrin beta chain, non-erythrocytic 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTTTVATDYD	NIEIQQQYSD	VNNRWDVDDW	DNENSSARLF	ERSRIKALAD
60	70	80	90	100
EREAVQKKTF	TKWVNSHLAR	VSCRITDLYT	DLRDGRMLIK	LLEVLSGERL
110	120	130	140	150
PKPTKGRMRI	HCLENVDKAL	QFLKEQRVHL	ENMGSHDIVD	GNHRLTLGLI
160	170	180	190	200
WTIILRFQIQ	DISVETEDNK	EKKSAKDALL	LWCQMKTAGY	PNVNIHNFTT
210	220	230	240	250
SWRDGMAFNA	LIHKHRPDLI	DFDKLKKSNA	HYNLQNAFNL	AEQHLGLTKL
260	270	280	290	300
LDPEDISVDH	PDEKSIITYV	VTYYHYFSKM	KALAVEGKRI	GKVLDNAIET
310	320	330	340	350
EKMIEKYESL	ASDLLEWIEQ	TIIILNNRKF	ANSLVGVQQQ	LQAFNTYRTV
360	370	380	390	400
EKPPKFTEKG	NLEVLLFTIQ	SKMRANNQKV	YMPREGKLIS	DINKAWERLE
410	420	430	440	450
KAEHERELAL	RNELIRQEKL	EQLARRFDRK	AAMRETWLSE	NQRLVSQDNF
460	470	480	490	500
GFDLPAVEAA	TKKHEAIETD	IAAYEERVQA	VVAVARELEA	ENYHDIKRIT
510	520	530	540	550
ARKDNVIRLW	EYLLELLRAR	RQRLEMNLGL	QKIFQEMLYI	MDWMDEMKVL
560	570	580	590	600
VLSQDYGKHL	LGVEDLLQKH	TLVEADIGIQ	AERVRGVNAS	AQKFATDGEG
610	620	630	640	650
YKPCDPQVIR	DRVAHMEFCY	QELCQLAAER	RARLEESRRL	WKFFWEMAEE
660	670	680	690	700
EGWIREKEKI	LSSDDYGKDL	TSVMRLLSKH	RAFEDEMSGR	SGHFEQAIKE
710	720	730	740	750
GEDMIAEEHF	GSEKIRERII	YIREQWANLE	QLSAIRKKRL	EEASLLHQFQ
760	770	780	790	800
ADADDIDAWM	LDILKIVSSS	DVGHDEYSTQ	SLVKKHKDVA	EEIANYRPTL
810	820	830	840	850
DTLHEQASAL	PQEHAESPDV	RGRLSGIEER	YKEVAELTRL	RKQALQDTLA
860	870	880	890	900
LYKMFSEADA	CELWIDEKEQ	WLNNMQIPEK	LEDLEVIQHR	FESLEPEMNN
910	920	930	940	950
QASRVAVVNQ	IARQLMHSGH	PSEKEIKAQQ	DKLNTRWSQF	RELVDRKKDA
960	970	980	990	1000
LLSALSIQNY	HLECNETKSW	IREKTKVIES	TQDLGNDLAG	VMALQRKLTG
1010	1020	1030	1040	1050
MERDLVAIEA	KLSDLQKEAE	KLESEHPDQA	QAILSRLAEI	SDVWEEMKTT
1060	1070	1080	1090	1100
LKNREASLGE	ASKLQQFLRD	LDDFQSWLSR	TQTAIASEDM	PNTLTEAEKL
1110	1120	1130	1140	1150
LTQHENIKNE	IDNYEEDYQK	MRDMGEMVTQ	GQTDAQYMFL	RQRLQALDTG
1160	1170	1180	1190	1200
WNELHKMWEN	RQNLLSQSHA	YQQFLRDTKQ	AEAFLNNQEY	VLAHTEMPTT
1210	1220	1230	1240	1250
LEGAEAAIKK	QEDFMTTMDA	NEEKINAVVE	TGRRLVSDGN	INSDRIQEKV
1260	1270	1280	1290	1300
DSIDDRHRKN	RETASELLMR	LKDNRDLQKF	LQDCQELSLW	INEKMLTAQD
1310	1320	1330	1340	1350
MSYDEARNLH	SKWLKHQAFM	AELASNKEWL	DKIEKEGMQL	ISEKPETEAV
1360	1370	1380	1390	1400
VKEKLTGLHK	MWEVLESTTQ	TKAQRLFDAN	KAELFTQSCA	DLDKWLHGLE
1410	1420	1430	1440	1450
SQIQSDDYGK	DLTSVNILLK	KQQMLENQME	VRKKEIEELQ	SQAQALSQEG
1460	1470	1480	1490	1500
KSTDEVDSKR	LTVQTKFMEL	LEPLNERKHN	LLASKEIHQF	NRDVEDEILW
1510	1520	1530	1540	1550
VGERMPLATS	TDHGHNLQTV	QLLIKKNQTL	QKEIQGHQPR	IDDIFERSQN
1560	1570	1580	1590	1600
IVTDSSSLSA	EAIRQRLADL	KQLWGLLIEE	TEKRHRRLEE	AHRAQQYYFD
1610	1620	1630	1640	1650
AAEAEAWMSE	QELYMMSEEK	AKDEQSAVSM	LKKHQILEQA	VEDYAETVHQ
1660	1670	1680	1690	1700
LSKTSRALVA	DSHPESERIS	MRQSKVDKLY	AGLKDLAEER	RGKLDERHRL
1710	1720	1730	1740	1750
FQLNREVDDL	EQWIAEREVV	AGSHELGQDY	EHVTMLQERF	REFARDTGNI
1760	1770	1780	1790	1800
GQERVDTVNH	LADELINSGH	SDAATIAEWK	DGLNEAWADL	LELIDTRTQI
1810	1820	1830	1840	1850
LAASYELHKF	YHDAKEIFGR	IQDKHKKLPE	ELGRDQNTVE	TLQRMHTTFE
1860	1870	1880	1890	1900
HDIQALGTQV	RQLQEDAARL	QAAYAGDKAD	DIQKRENEVL	EAWKSLLDAC
1910	1920	1930	1940	1950
ESRRVRLVDT	GDKFRFFSMV	RDLMLWMEDV	IRQIEAQEKP	RDVSSVELLM
1960	1970	1980	1990	2000
NNHQGIKAEI	DARNDSFTTC	IELGKSLLAR	KHYASEEIKE	KLLQLTEKRK
2010	2020	2030	2040	2050
EMIDKWEDRW	EWLRLILEVH	QFSRDASVAE	AWLLGQEPYL	SSREIGQSVD
2060	2070	2080	2090	2100
EVEKLIKRHE	AFEKSAATWD	ERFSALERLT	TLELLEVRRQ	QEEEERKRRP
2110	2120	2130	2140	2150
PSPEPSTKVS	EEAESQQQWD	TSKGEQVSQN	GLPAEQGSPR	MAETVDTSEM
2160	2170	2180	2190	2200
VNGATEQRTS	SKESSPIPSP	TSDRKAKTAL	PAQSAATLPA	RTQETPSAQM
2210	2220	2230	2240	2250
EGFLNRKHEW	EAHNKKASSR	SWHNVYCVIN	NQEMGFYKDA	KTAASGIPYH
2260	2270	2280	2290	2300
SEVPVSLKEA	VCEVALDYKK	KKHVFKLRLN	DGNEYLFQAK	DDEEMNTWIQ
2310	2320	2330	2340	2350
AISSAISSDK	HEVSASTQST	PASSRAQTLP	TSVVTITSES	SPGKREKDKE
2360	2364
KDKEKRFSLF	GKKK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-590 Collapse assay details

Data source: Panorama

Official Gene Symbol: SPTBN1
Peptide Sequence: DVEDEILWVGER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 1493
Peptide End: 1504

CPTAC ID: CPTAC-590
Peptide Molecular Mass: 1,458.6991
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI: Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.