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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MCM6

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MCM6 N/A
Sequence Length (AA) Molecular Weight (Da)
821 92889
Protein Name
DNA replication licensing factor MCM6
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDLAAAAEPG AGSQHLEVRD EVAEKCQKLF LDFLEEFQSS DGEIKYLQLA
60 70 80 90 100
EELIRPERNT LVVSFVDLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD
110 120 130 140 150
RKEIPLAKDF YVAFQDLPTR HKIRELTSSR IGLLTRISGQ VVRTHPVHPE
160 170 180 190 200
LVSGTFLCLD CQTVIRDVEQ QFKYTQPNIC RNPVCANRRR FLLDTNKSRF
210 220 230 240 250
VDFQKVRIQE TQAELPRGSI PRSLEVILRA EAVESAQAGD KCDFTGTLIV
260 270 280 290 300
VPDVSKLSTP GARAETNSRV SGVDGYETEG IRGLRALGVR DLSYRLVFLA
310 320 330 340 350
CCVAPTNPRF GGKELRDEEQ TAESIKNQMT VKEWEKVFEM SQDKNLYHNL
360 370 380 390 400
CTSLFPTIHG NDEVKRGVLL MLFGGVPKTT GEGTSLRGDI NVCIVGDPST
410 420 430 440 450
AKSQFLKHVE EFSPRAVYTS GKASSAAGLT AAVVRDEESH EFVIEAGALM
460 470 480 490 500
LADNGVCCID EFDKMDVRDQ VAIHEAMEQQ TISITKAGVK ATLNARTSIL
510 520 530 540 550
AAANPISGHY DRSKSLKQNI NLSAPIMSRF DLFFILVDEC NEVTDYAIAR
560 570 580 590 600
RIVDLHSRIE ESIDRVYSLD DIRRYLLFAR QFKPKISKES EDFIVEQYKH
610 620 630 640 650
LRQRDGSGVT KSSWRITVRQ LESMIRLSEA MARMHCCDEV QPKHVKEAFR
660 670 680 690 700
LLNKSIIRVE TPDVNLDQEE EIQMEVDEGA GGINGHADSP APVNGINGYN
710 720 730 740 750
EDINQESAPK ASLRLGFSEY CRISNLIVLH LRKVEEEEDE SALKRSELVN
760 770 780 790 800
WYLKEIESEI DSEEELINKK RIIEKVIHRL THYDHVLIEL TQAGLKGSTE
810 820 821
GSESYEEDPY LVVNPNYLLE D

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5918 Collapse assay details

Data source: Panorama

Official Gene Symbol
MCM6
Peptide Sequence
EIESEIDSEEELINK
Modification Type
unmodified
Protein - Site of Modification
755
Peptide - Site of Modification
N/A
Peptide Start
755
Peptide End
769
CPTAC ID
CPTAC-5918
Peptide Molecular Mass
1,775.8313
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (2+) 113.9 66 62.6 125.4 73.7 64.1 169.4 98.9 89.6 15 26 15
b7 (1+) 152.3 58.4 25.9 162.1 55.3 27.8 222.4 80.4 38 15 26 15
b6 (1+) 66.1 54.1 34.7 77.2 62.3 48.2 101.6 82.5 59.4 15 27 15
y2 (1+) 77.9 71.6 29.4 78.8 85.6 22.5 110.8 111.6 37 15 27 15
y5 (1+) 87 49.3 26.3 79.5 48.2 30.2 117.9 68.9 40 15 27 15
y6 (1+) 46.3 78.6 34.3 85.3 64.5 37.4 97.1 101.7 50.7 15 27 15
b12 (2+) 105.9 77.5 43.1 87.7 70.2 39.9 137.5 104.6 58.7 15 27 15
sum 29.1 37.4 12.8 47.8 44.9 9.9 56 58.4 16.2 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-5919 Collapse assay details

Data source: Panorama

Official Gene Symbol
MCM6
Peptide Modified Sequence
EIESEIDS[+79.966331]EEELINK
Modification Type
Phospho (ST)
Protein - Site of Modification
763
Peptide - Site of Modification
8
Peptide Start
755
Peptide End
769
CPTAC ID
CPTAC-5919
Peptide Molecular Mass
1,855.7976
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10-98 (1+) 74.4 34.2 22.8 84.1 36.4 22 112.3 49.9 31.7 15 27 15
y9 (1+) 35.5 23.2 31.1 47.2 30.4 34.4 59.1 38.2 46.4 15 27 15
y9-98 (1+) 47.8 28.7 29.2 55.1 34.2 25.5 72.9 44.6 38.8 15 27 15
y8 (1+) 75.6 35.3 22.4 81.4 45.3 29.6 111.1 57.4 37.1 15 27 15
y5 (1+) 61.3 29.6 23.1 87.6 37.4 31.3 106.9 47.7 38.9 15 27 15
y4 (1+) 66 19.7 22.4 68 30.4 23.5 94.8 36.2 32.5 15 27 15
b5 (1+) 65.7 28 14.6 64.6 25.2 21.2 92.1 37.7 25.7 15 27 15
z9-98 (1+) 74.6 36.7 41.8 77.5 40.9 51.9 107.6 55 66.6 15 27 15
sum 13.6 12.6 11 33 20.3 13.6 35.7 23.9 17.5 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments