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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

NUMA1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
NUMA1 NMP22, NUMA
Sequence Length (AA) Molecular Weight (Da)
2115 238260
Protein Name
Nuclear mitotic apparatus protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTLHATRGAA LLSWVNSLHV ADPVEAVLQL QDCSIFIKII DRIHGTEEGQ
60 70 80 90 100
QILKQPVSER LDFVCSFLQK NRKHPSSPEC LVSAQKVLEG SELELAKMTM
110 120 130 140 150
LLLYHSTMSS KSPRDWEQFE YKIQAELAVI LKFVLDHEDG LNLNEDLENF
160 170 180 190 200
LQKAPVPSTC SSTFPEELSP PSHQAKREIR FLELQKVASS SSGNNFLSGS
210 220 230 240 250
PASPMGDILQ TPQFQMRRLK KQLADERSNR DELELELAEN RKLLTEKDAQ
260 270 280 290 300
IAMMQQRIDR LALLNEKQAA SPLEPKELEE LRDKNESLTM RLHETLKQCQ
310 320 330 340 350
DLKTEKSQMD RKINQLSEEN GDLSFKLREF ASHLQQLQDA LNELTEEHSK
360 370 380 390 400
ATQEWLEKQA QLEKELSAAL QDKKCLEEKN EILQGKLSQL EEHLSQLQDN
410 420 430 440 450
PPQEKGEVLG DVLQLETLKQ EAATLAANNT QLQARVEMLE TERGQQEAKL
460 470 480 490 500
LAERGHFEEE KQQLSSLITD LQSSISNLSQ AKEELEQASQ AHGARLTAQV
510 520 530 540 550
ASLTSELTTL NATIQQQDQE LAGLKQQAKE KQAQLAQTLQ QQEQASQGLR
560 570 580 590 600
HQVEQLSSSL KQKEQQLKEV AEKQEATRQD HAQQLATAAE EREASLRERD
610 620 630 640 650
AALKQLEALE KEKAAKLEIL QQQLQVANEA RDSAQTSVTQ AQREKAELSR
660 670 680 690 700
KVEELQACVE TARQEQHEAQ AQVAELELQL RSEQQKATEK ERVAQEKDQL
710 720 730 740 750
QEQLQALKES LKVTKGSLEE EKRRAADALE EQQRCISELK AETRSLVEQH
760 770 780 790 800
KRERKELEEE RAGRKGLEAR LQQLGEAHQA ETEVLRRELA EAMAAQHTAE
810 820 830 840 850
SECEQLVKEV AAWRERYEDS QQEEAQYGAM FQEQLMTLKE ECEKARQELQ
860 870 880 890 900
EAKEKVAGIE SHSELQISRQ QNELAELHAN LARALQQVQE KEVRAQKLAD
910 920 930 940 950
DLSTLQEKMA ATSKEVARLE TLVRKAGEQQ ETASRELVKE PARAGDRQPE
960 970 980 990 1000
WLEEQQGRQF CSTQAALQAM EREAEQMGNE LERLRAALME SQGQQQEERG
1010 1020 1030 1040 1050
QQEREVARLT QERGRAQADL ALEKAARAEL EMRLQNALNE QRVEFATLQE
1060 1070 1080 1090 1100
ALAHALTEKE GKDQELAKLR GLEAAQIKEL EELRQTVKQL KEQLAKKEKE
1110 1120 1130 1140 1150
HASGSGAQSE AAGRTEPTGP KLEALRAEVS KLEQQCQKQQ EQADSLERSL
1160 1170 1180 1190 1200
EAERASRAER DSALETLQGQ LEEKAQELGH SQSALASAQR ELAAFRTKVQ
1210 1220 1230 1240 1250
DHSKAEDEWK AQVARGRQEA ERKNSLISSL EEEVSILNRQ VLEKEGESKE
1260 1270 1280 1290 1300
LKRLVMAESE KSQKLEERLR LLQAETASNS ARAAERSSAL REEVQSLREE
1310 1320 1330 1340 1350
AEKQRVASEN LRQELTSQAE RAEELGQELK AWQEKFFQKE QALSTLQLEH
1360 1370 1380 1390 1400
TSTQALVSEL LPAKHLCQQL QAEQAAAEKR HREELEQSKQ AAGGLRAELL
1410 1420 1430 1440 1450
RAQRELGELI PLRQKVAEQE RTAQQLRAEK ASYAEQLSML KKAHGLLAEE
1460 1470 1480 1490 1500
NRGLGERANL GRQFLEVELD QAREKYVQEL AAVRADAETR LAEVQREAQS
1510 1520 1530 1540 1550
TARELEVMTA KYEGAKVKVL EERQRFQEER QKLTAQVEQL EVFQREQTKQ
1560 1570 1580 1590 1600
VEELSKKLAD SDQASKVQQQ KLKAVQAQGG ESQQEAQRLQ AQLNELQAQL
1610 1620 1630 1640 1650
SQKEQAAEHY KLQMEKAKTH YDAKKQQNQE LQEQLRSLEQ LQKENKELRA
1660 1670 1680 1690 1700
EAERLGHELQ QAGLKTKEAE QTCRHLTAQV RSLEAQVAHA DQQLRDLGKF
1710 1720 1730 1740 1750
QVATDALKSR EPQAKPQLDL SIDSLDLSCE EGTPLSITSK LPRTQPDGTS
1760 1770 1780 1790 1800
VPGEPASPIS QRLPPKVESL ESLYFTPIPA RSQAPLESSL DSLGDVFLDS
1810 1820 1830 1840 1850
GRKTRSARRR TTQIINITMT KKLDVEEPDS ANSSFYSTRS APASQASLRA
1860 1870 1880 1890 1900
TSSTQSLARL GSPDYGNSAL LSLPGYRPTT RSSARRSQAG VSSGAPPGRN
1910 1920 1930 1940 1950
SFYMGTCQDE PEQLDDWNRI AELQQRNRVC PPHLKTCYPL ESRPSLSLGT
1960 1970 1980 1990 2000
ITDEEMKTGD PQETLRRASM QPIQIAEGTG ITTRQQRKRV SLEPHQGPGT
2010 2020 2030 2040 2050
PESKKATSCF PRPMTPRDRH EGRKQSTTEA QKKAAPASTK QADRRQSMAF
2060 2070 2080 2090 2100
SILNTPKKLG NSLLRRGASK KALSKASPNT RSGTRRSPRI ATTTASAATA
2110 2115
AAIGATPRAK GKAKH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5920 Collapse assay details

Data source: Panorama

Official Gene Symbol
NUMA1
Peptide Modified Sequence
LSQLEEHLS[+79.966331]QLQDNPPQEK
Modification Type
Phospho (ST)
Protein - Site of Modification
396
Peptide - Site of Modification
9
Peptide Start
387
Peptide End
405
CPTAC ID
CPTAC-5920
Peptide Molecular Mass
2,312.0686
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 17.7 10.8 7.5 39.5 14.6 8.4 43.3 18.2 11.3 15 27 15
y7 (1+) 20.6 10.8 5.8 29.3 16 11.8 35.8 19.3 13.1 15 27 15
y8 (1+) 52 15.1 6.7 51.5 20.9 9.5 73.2 25.8 11.6 15 27 15
b14 (2+) 23.7 13.1 7 25.4 15.5 8.8 34.7 20.3 11.2 15 27 15
y5 (1+) 8.1 7.1 7.9 12.5 11.9 8.9 14.9 13.9 11.9 15 27 15
y4 (1+) 19.8 8.2 11.3 26.5 14.2 11.2 33.1 16.4 15.9 15 27 15
y16-98 (2+) 36.1 15.8 6.1 47.4 18.6 10.6 59.6 24.4 12.2 15 27 15
sum 5.8 4.7 3.9 11 11.7 7.5 12.4 12.6 8.5 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-937 Collapse assay details

Data source: Panorama

Official Gene Symbol
NUMA1
Peptide Modified Sequence
VSLEPHQGPGT[+80.0]PESK
Modification Type
Phospho (ST)
Protein - Site of Modification
2000
Peptide - Site of Modification
11
Peptide Start
1990
Peptide End
2004
CPTAC ID
CPTAC-937
Peptide Molecular Mass
1,641.7400
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 15.2 7.5 6.9 23 7.6 8 27.6 10.7 10.6 15 24 15
y13 (2+) 10.1 6.6 6.8 24 7 8 26 9.6 10.5 15 24 15
y14 (2+) 13 8.3 8.7 26.3 9.2 10.7 29.3 12.4 13.8 15 24 15
sum 11.8 6.8 7.3 24.2 7.2 8.8 26.9 9.9 11.4 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments