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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

CDCA8

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDCA8 PESCRG3
Sequence Length (AA) Molecular Weight (Da)
280 31323
Protein Name
Borealin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPRKGSSRV AKTNSLRRRK LASFLKDFDR EVEIRIKQIE SDRQNLLKEV
60 70 80 90 100
DNLYNIEILR LPKALREMNW LDYFALGGNK QALEEAATAD LDITEINKLT
110 120 130 140 150
AEAIQTPLKS AKTRKVIQVD EMIVEEEEEE ENERKNLQTA RVKRCPPSKK
160 170 180 190 200
RTQSIQGKGK GKRSSRANTV TPAVGRLEVS MVKPTPGLTP RFDSRVFKTP
210 220 230 240 250
GLRTPAAGER IYNISGNGSP LADSKEIFLT VPVGGGESLR LLASDLQRHS
260 270 280
IAQLDPEALG NIKKLSNRLA QICSSIRTHK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5921 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDCA8
Peptide Modified Sequence
LTAEAIQT[+79.966331]PLK
Modification Type
Phospho (ST)
Protein - Site of Modification
107
Peptide - Site of Modification
8
Peptide Start
99
Peptide End
109
CPTAC ID
CPTAC-5921
Peptide Molecular Mass
1,263.6476
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 24.7 8.2 7.7 27.6 12.6 11.8 37 15 14.1 15 27 15
y7 (1+) 12 9 7.2 16.9 13 11.2 20.7 15.8 13.3 15 27 15
y8 (1+) 27.5 9.5 11.1 40.1 16.2 15.2 48.6 18.8 18.8 15 27 15
y9 (1+) 13 5.2 7.4 27.7 20.6 14.7 30.6 21.2 16.5 15 27 15
b5 (1+) 24.3 9.6 7.8 28.8 13 13.6 37.7 16.2 15.7 15 27 15
y9 (2+) 30.6 11.4 6.4 25.8 14.6 14.3 40 18.5 15.7 15 27 15
y6 (1+) 25.9 10.5 6.3 30.2 15.8 13.6 39.8 19 15 15 27 15
sum 7 3.2 3.5 17.6 12.3 10.7 18.9 12.7 11.3 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments