NCAPH2, CPTAC-5922 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NCAPH2	CAPH2

Sequence Length (AA)	Molecular Weight (Da)
605	68227

Protein Name
Condensin-2 complex subunit H2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEDVEARFAH	LLQPIRDLTK	NWEVDVAAQL	GEYLEELDQI	CISFDEGKTT
60	70	80	90	100
MNFIEAALLI	QGSACVYSKK	VEYLYSLVYQ	ALDFISGKRR	AKQLSSVQED
110	120	130	140	150
RANGVASSGV	PQEAENEFLS	LDDFPDSRTN	VDLKNDQTPS	EVLIIPLLPM
160	170	180	190	200
ALVAPDEMEK	NNNPLYSRQG	EVLASRKDFR	MNTCVPHPRG	AFMLEPEGMS
210	220	230	240	250
PMEPAGVSPM	PGTQKDTGRT	EEQPMEVSVC	RSPVPALGFS	QEPGPSPEGP
260	270	280	290	300
MPLGGGEDED	AEEAVELPEA	SAPKAALEPK	ESRSPQQSAA	LPRRYMLRER
310	320	330	340	350
EGAPEPASCV	KETPDPWQSL	DPFDSLESKP	FKKGRPYSVP	PCVEEALGQK
360	370	380	390	400
RKRKGAAKLQ	DFHQWYLAAY	ADHADSRRLR	RKGPSFADME	VLYWTHVKEQ
410	420	430	440	450
LETLRKLQRR	EVAEQWLRPA	EEDHLEDSLE	DLGAADDFLE	PEEYMEPEGA
460	470	480	490	500
DPREAADLDA	VPMSLSYEEL	VRRNVELFIA	TSQKFVQETE	LSQRIRDWED
510	520	530	540	550
TVQPLLQEQE	QHVPFDIHTY	GDQLVSRFPQ	LNEWCPFAEL	VAGQPAFEVC
560	570	580	590	600
RSMLASLQLA	NDYTVEITQQ	PGLEMAVDTM	SLRLLTHQRA	HKRFQTYAAP
605
SMAQP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5922 Collapse assay details

Data source: Panorama

Official Gene Symbol: NCAPH2
Peptide Modified Sequence: FVQETELS[+79.966331]QR
Modification Type: Phospho (ST)
Protein - Site of Modification: 493
Peptide - Site of Modification: 8
Peptide Start: 485
Peptide End: 494

CPTAC ID: CPTAC-5922
Peptide Molecular Mass: 1,315.5809
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: Cell Lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Sciex 5500 QTRAP
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent 425
Column Packing: Reprosil
Column Dimensions: 75um x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	42.4	12.7	9	29.8	13.3	14.8	51.8	18.4	17.3	15	27	15
y8 (2+)	35.4	13.4	3.8	42.7	18.4	12.1	55.5	22.8	12.7	15	27	15
y4-98 (1+)	26.8	10.2	7.2	34.6	16	12.4	43.8	19	14.3	15	27	15
y6-98 (1+)	32.9	9.4	4.8	35.4	14.6	15.2	48.3	17.4	15.9	15	27	15
y8-98 (1+)	33.2	12.1	9.1	36.4	14.3	12.3	49.3	18.7	15.3	15	27	15
y7-98 (1+)	36.3	12.4	5.2	31.8	12.3	10.2	48.3	17.5	11.4	15	27	15
b3 (1+)	51	12	6.6	46.5	15.6	12.5	69	19.7	14.1	15	27	15
sum	11.5	5	4.4	15.8	10	11	19.5	11.2	11.8	15	27	15

NCAPH2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5922 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

NCAPH2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5922 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments