CD74, CPTAC-5941 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CD74	DHLAG

Sequence Length (AA)	Molecular Weight (Da)
296	33516

Protein Name
HLA class II histocompatibility antigen gamma chain

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MHRRRSRSCR	EDQKPVMDDQ	RDLISNNEQL	PMLGRRPGAP	ESKCSRGALY
60	70	80	90	100
TGFSILVTLL	LAGQATTAYF	LYQQQGRLDK	LTVTSQNLQL	ENLRMKLPKP
110	120	130	140	150
PKPVSKMRMA	TPLLMQALPM	GALPQGPMQN	ATKYGNMTED	HVMHLLQNAD
160	170	180	190	200
PLKVYPPLKG	SFPENLRHLK	NTMETIDWKV	FESWMHHWLL	FEMSRHSLEQ
210	220	230	240	250
KPTDAPPKVL	TKCQEEVSHI	PAVHPGSFRP	KCDENGNYLP	LQCYGSIGYC
260	270	280	290	296
WCVFPNGTEV	PNTRSRGHHN	CSESLELEDP	SSGLGVTKQD	LGPVPM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5941 Collapse assay details

Data source: Panorama

Official Gene Symbol: CD74
Peptide Modified Sequence: C[+57.021464]QEEVSHIPAVHPGSFRPK
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 213
Peptide End: 231

CPTAC ID: CPTAC-5941
Peptide Molecular Mass: 2,174.0691
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (2+)	73.9	53.5	7.4	76.9	47.3	10.2	106.7	71.4	12.6	13	25	14
y14 (2+)	84.4	74	27.6	94.1	81	27	126.4	109.7	38.6	13	25	14
y16 (2+)	156.4	103	52.6	218.9	82.9	64.7	269	132.2	83.4	13	25	14
y17 (2+)	137.5	72.1	14.6	133	68.7	23.5	191.3	99.6	27.7	13	25	14
y7 (1+)	87.4	31.3	22.1	75.1	30	34.3	115.2	43.4	40.8	13	25	14
y11 (1+)	49.2	30.7	14.8	46.6	25.1	16.8	67.8	39.7	22.4	13	25	14
y7 (2+)	112.1	82.3	23.5	144.2	84.2	32.7	182.6	117.7	40.3	13	24	13
sum	39.2	22.5	10.5	39.5	22.9	14.9	55.6	32.1	18.2	13	25	14

CD74

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5941 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CD74

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5941 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments