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Overview Data source: UniProt

Official Gene Symbol Other Aliases
STOML2 SLP2
Sequence Length (AA) Molecular Weight (Da)
356 38534
Protein Name
Stomatin-like protein 2, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLARAARGTG ALLLRGSLLA SGRAPRRASS GLPRNTVVLF VPQQEAWVVE
60 70 80 90 100
RMGRFHRILE PGLNILIPVL DRIRYVQSLK EIVINVPEQS AVTLDNVTLQ
110 120 130 140 150
IDGVLYLRIM DPYKASYGVE DPEYAVTQLA QTTMRSELGK LSLDKVFRER
160 170 180 190 200
ESLNASIVDA INQAADCWGI RCLRYEIKDI HVPPRVKESM QMQVEAERRK
210 220 230 240 250
RATVLESEGT RESAINVAEG KKQAQILASE AEKAEQINQA AGEASAVLAK
260 270 280 290 300
AKAKAEAIRI LAAALTQHNG DAAASLTVAE QYVSAFSKLA KDSNTILLPS
310 320 330 340 350
NPGDVTSMVA QAMGVYGALT KAPVPGTPDS LSSGSSRDVQ GTDASLDEEL
356
DRVKMS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-595 Collapse assay details

Data source: Panorama

Official Gene Symbol
STOML2
Peptide Sequence
AEQINQAAGEASAVLAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
234
Peptide End
250
CPTAC ID
CPTAC-595
Peptide Molecular Mass
1,669.8635
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (1+) 8.1 7.9 6.9 14 13 8.5 16.2 15.2 10.9 15 25 15
y10 (1+) 11.9 6.3 9.9 18.3 14.2 10.2 21.8 15.5 14.2 15 25 15
y9 (1+) 13.1 8.4 9 15.5 14.6 16 20.3 16.8 18.4 15 25 15
sum 8.7 5.5 6.8 13.6 13.1 10.5 16.1 14.2 12.5 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-596 Collapse assay details

Data source: Panorama

Official Gene Symbol
STOML2
Peptide Sequence
APVPGTPDSLSSGSSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
322
Peptide End
337
CPTAC ID
CPTAC-596
Peptide Molecular Mass
1,513.7373
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 8.4 6.7 4.5 10.1 7.5 5.8 13.1 10.1 7.3 15 25 15
y8 (1+) 19.6 8.5 9.1 19.7 6.7 12.5 27.8 10.8 15.5 15 25 15
y13 (2+) 6.4 4.3 4.4 7.2 5.1 5.1 9.6 6.7 6.7 15 25 15
sum 4.5 4.3 4.3 5.9 5.1 4.5 7.4 6.7 6.2 15 25 15

Additional Resources and Comments