CD33, CPTAC-5951 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CD33	SIGLEC3

Sequence Length (AA)	Molecular Weight (Da)
364	39825

Protein Name
Myeloid cell surface antigen CD33

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPLLLLLPLL	WAGALAMDPN	FWLQVQESVT	VQEGLCVLVP	CTFFHPIPYY
60	70	80	90	100
DKNSPVHGYW	FREGAIISRD	SPVATNKLDQ	EVQEETQGRF	RLLGDPSRNN
110	120	130	140	150
CSLSIVDARR	RDNGSYFFRM	ERGSTKYSYK	SPQLSVHVTD	LTHRPKILIP
160	170	180	190	200
GTLEPGHSKN	LTCSVSWACE	QGTPPIFSWL	SAAPTSLGPR	TTHSSVLIIT
210	220	230	240	250
PRPQDHGTNL	TCQVKFAGAG	VTTERTIQLN	VTYVPQNPTT	GIFPGDGSGK
260	270	280	290	300
QETRAGVVHG	AIGGAGVTAL	LALCLCLIFF	IVKTHRRKAA	RTAVGRNDTH
310	320	330	340	350
PTTGSASPKH	QKKSKLHGPT	ETSSCSGAAP	TVEMDEELHY	ASLNFHGMNP
360	364
SKDTSTEYSE	VRTQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5951 Collapse assay details

Data source: Panorama

Official Gene Symbol: CD33
Peptide Sequence: ILIPGTLEPGHSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 147
Peptide End: 159

CPTAC ID: CPTAC-5951
Peptide Molecular Mass: 1,360.7715
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (2+)	8.6	3.6	3.2	13.1	13.7	16.7	15.7	14.2	17	13	25	14
b6 (1+)	7	6	3.2	9.7	12	13	12	13.4	13.4	13	25	14
b7 (1+)	10.8	5.2	5.6	12.2	12.1	14.5	16.3	13.2	15.5	13	25	14
y6 (1+)	6.8	6.1	4.6	13.4	20.5	22.6	15	21.4	23.1	13	25	14
y5 (1+)	10.5	5.1	2.6	13.1	14.1	17	16.8	15	17.2	13	25	14
y12 (2+)	14.1	4.9	5	17.1	14.2	16	22.2	15	16.8	13	25	14
y10 (2+)	5.5	4.8	4.7	7.7	13.4	14	9.5	14.2	14.8	13	25	14
sum	7	3.9	3.3	10.2	13.7	15.5	12.4	14.2	15.8	13	25	14

CD33

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5951 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CD33

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5951 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments