FAS, CPTAC-5953 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FAS	APT1, FAS1, TNFRSF6

Sequence Length (AA)	Molecular Weight (Da)
335	37732

Protein Name
Tumor necrosis factor receptor superfamily member 6

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLGIWTLLPL	VLTSVARLSS	KSVNAQVTDI	NSKGLELRKT	VTTVETQNLE
60	70	80	90	100
GLHHDGQFCH	KPCPPGERKA	RDCTVNGDEP	DCVPCQEGKE	YTDKAHFSSK
110	120	130	140	150
CRRCRLCDEG	HGLEVEINCT	RTQNTKCRCK	PNFFCNSTVC	EHCDPCTKCE
160	170	180	190	200
HGIIKECTLT	SNTKCKEEGS	RSNLGWLCLL	LLPIPLIVWV	KRKEVQKTCR
210	220	230	240	250
KHRKENQGSH	ESPTLNPETV	AINLSDVDLS	KYITTIAGVM	TLSQVKGFVR
260	270	280	290	300
KNGVNEAKID	EIKNDNVQDT	AEQKVQLLRN	WHQLHGKKEA	YDTLIKDLKK
310	320	330	335
ANLCTLAEKI	QTIILKDITS	DSENSNFRNE	IQSLV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5953 Collapse assay details

Data source: Panorama

Official Gene Symbol: FAS
Peptide Sequence: EAYDTLIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 289
Peptide End: 296

CPTAC ID: CPTAC-5953
Peptide Molecular Mass: 951.4913
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	14.6	4.6	9.1	12.3	12	10.9	19.1	12.9	14.2	13	25	14
y5 (1+)	6.7	9.5	7.8	7.8	10.4	10.6	10.3	14.1	13.2	13	25	14
y4 (1+)	19.6	6.9	7.7	17.2	10.1	10.8	26.1	12.2	13.3	13	25	14
y6 (2+)	14.4	11.9	6.4	12.8	10.7	10.5	19.3	16	12.3	13	25	14
sum	9.3	5.2	5.6	9.6	7.8	9.5	13.4	9.4	11	13	25	14

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments