TNFRSF17, CPTAC-5971 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TNFRSF17	BCM, BCMA

Sequence Length (AA)	Molecular Weight (Da)
184	20165

Protein Name
Tumor necrosis factor receptor superfamily member 17

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLQMAGQCSQ	NEYFDSLLHA	CIPCQLRCSS	NTPPLTCQRY	CNASVTNSVK
60	70	80	90	100
GTNAILWTCL	GLSLIISLAV	FVLMFLLRKI	NSEPLKDEFK	NTGSGLLGMA
110	120	130	140	150
NIDLEKSRTG	DEIILPRGLE	YTVEECTCED	CIKSKPKVDS	DHCFPLPAME
160	170	180	184
EGATILVTTK	TNDYCKSLPA	ALSATEIEKS	ISAR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-5934 Collapse assay details

Data source: Panorama

Official Gene Symbol: TNFRSF17
Peptide Sequence: SLPAALSATEIEK
Modification Type: unmodified
Protein - Site of Modification: 167
Peptide - Site of Modification: N/A
Peptide Start: 167
Peptide End: 179

CPTAC ID: CPTAC-5934
Peptide Molecular Mass: 1,328.7187
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: Cell Lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Sciex 5500 QTRAP
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent 425
Column Packing: Reprosil
Column Dimensions: 75um x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b6 (1+)	42.4	30.4	12.5	43.5	30.9	19.8	60.7	43.3	23.4	15	27	15
b5 (1+)	103.9	27.5	21.7	98.8	30.4	25.6	143.4	41	33.6	15	27	15
y11 (2+)	19.9	9.4	6.2	27.5	14.4	10.1	33.9	17.2	11.9	15	27	15
y6 (1+)	60.6	30.7	14.6	82.2	31.4	13.6	102.1	43.9	20	15	27	15
y7 (1+)	57.8	19.3	6.1	46.1	22.5	10.7	73.9	29.6	12.3	15	27	15
y8 (1+)	69	21.4	17	75.5	24.4	22.6	102.3	32.5	28.3	15	27	15
y11 (1+)	48.5	18.3	12.3	54.6	20.8	13.4	73	27.7	18.2	15	27	15
sum	16.8	7.6	5.1	20.8	12.7	9.7	26.7	14.8	11	15	27	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments