VPS35, CPTAC-605 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
VPS35	MEM3

Sequence Length (AA)	Molecular Weight (Da)
796	91707

Protein Name
Vacuolar protein sorting-associated protein 35

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPTTQQSPQD	EQEKLLDEAI	QAVKVQSFQM	KRCLDKNKLM	DALKHASNML
60	70	80	90	100
GELRTSMLSP	KSYYELYMAI	SDELHYLEVY	LTDEFAKGRK	VADLYELVQY
110	120	130	140	150
AGNIIPRLYL	LITVGVVYVK	SFPQSRKDIL	KDLVEMCRGV	QHPLRGLFLR
160	170	180	190	200
NYLLQCTRNI	LPDEGEPTDE	ETTGDISDSM	DFVLLNFAEM	NKLWVRMQHQ
210	220	230	240	250
GHSRDREKRE	RERQELRILV	GTNLVRLSQL	EGVNVERYKQ	IVLTGILEQV
260	270	280	290	300
VNCRDALAQE	YLMECIIQVF	PDEFHLQTLN	PFLRACAELH	QNVNVKNIII
310	320	330	340	350
ALIDRLALFA	HREDGPGIPA	DIKLFDIFSQ	QVATVIQSRQ	DMPSEDVVSL
360	370	380	390	400
QVSLINLAMK	CYPDRVDYVD	KVLETTVEIF	NKLNLEHIAT	SSAVSKELTR
410	420	430	440	450
LLKIPVDTYN	NILTVLKLKH	FHPLFEYFDY	ESRKSMSCYV	LSNVLDYNTE
460	470	480	490	500
IVSQDQVDSI	MNLVSTLIQD	QPDQPVEDPD	PEDFADEQSL	VGRFIHLLRS
510	520	530	540	550
EDPDQQYLIL	NTARKHFGAG	GNQRIRFTLP	PLVFAAYQLA	FRYKENSKVD
560	570	580	590	600
DKWEKKCQKI	FSFAHQTISA	LIKAELAELP	LRLFLQGALA	AGEIGFENHE
610	620	630	640	650
TVAYEFMSQA	FSLYEDEISD	SKAQLAAITL	IIGTFERMKC	FSEENHEPLR
660	670	680	690	700
TQCALAASKL	LKKPDQGRAV	STCAHLFWSG	RNTDKNGEEL	HGGKRVMECL
710	720	730	740	750
KKALKIANQC	MDPSLQVQLF	IEILNRYIYF	YEKENDAVTI	QVLNQLIQKI
760	770	780	790	796
REDLPNLESS	EETEQINKHF	HNTLEHLRLR	RESPESEGPI	YEGLIL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-605 Collapse assay details

Data source: Panorama

Official Gene Symbol: VPS35
Peptide Sequence: IPVDTYNNILTVLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 404
Peptide End: 417

CPTAC ID: CPTAC-605
Peptide Molecular Mass: 1,601.9029
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI: Youngsoo Kim

Publication

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Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.