ADA, CPTAC-6227 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ADA	ADA1

Sequence Length (AA)	Molecular Weight (Da)
363	40764

Protein Name
Adenosine deaminase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAQTPAFDKP	KVELHVHLDG	SIKPETILYY	GRRRGIALPA	NTAEGLLNVI
60	70	80	90	100
GMDKPLTLPD	FLAKFDYYMP	AIAGCREAIK	RIAYEFVEMK	AKEGVVYVEV
110	120	130	140	150
RYSPHLLANS	KVEPIPWNQA	EGDLTPDEVV	ALVGQGLQEG	ERDFGVKARS
160	170	180	190	200
ILCCMRHQPN	WSPKVVELCK	KYQQQTVVAI	DLAGDETIPG	SSLLPGHVQA
210	220	230	240	250
YQEAVKSGIH	RTVHAGEVGS	AEVVKEAVDI	LKTERLGHGY	HTLEDQALYN
260	270	280	290	300
RLRQENMHFE	ICPWSSYLTG	AWKPDTEHAV	IRLKNDQANY	SLNTDDPLIF
310	320	330	340	350
KSTLDTDYQM	TKRDMGFTEE	EFKRLNINAA	KSSFLPEDEK	RELLDLLYKA
360	363
YGMPPSASAG	QNL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6227 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADA
Peptide Sequence: STLDTDYQMTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 302
Peptide End: 312

CPTAC ID: CPTAC-6227
Peptide Molecular Mass: 1,301.5809
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	9.2	3.2	4.2	9.1	4	5.9	12.9	5.1	7.2	15	15	15
y7 (1+)	8.7	3	4.8	11.2	4.6	7	14.2	5.5	8.5	15	15	15
y5 (1+)	9.9	3	4.6	13.1	5	8.5	16.4	5.8	9.7	15	15	15
y8 (1+)	6.5	3.4	3.9	11.3	5.5	7.6	13	6.5	8.5	15	15	15
sum	6.7	2.1	3.1	10.2	4.2	6.6	12.2	4.7	7.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for CPTAC-6234 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADA
Peptide Modified Sequence: STLDTDYQM[+15.994915]TK
Modification Type: Oxidation (M)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 302
Peptide End: 312

CPTAC ID: CPTAC-6234
Peptide Molecular Mass: 1,317.5758
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich