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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CXCL9 CMK, MIG, SCYB9
Sequence Length (AA) Molecular Weight (Da)
125 14019
Protein Name
C-X-C motif chemokine 9
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKKSGVLFLL GIILLVLIGV QGTPVVRKGR CSCISTNQGT IHLQSLKDLK
60 70 80 90 100
QFAPSPSCEK IEIIATLKNG VQTCLNPDSA DVKELIKKWE KQVSQKKKQK
110 120 125
NGKKHQKKKV LKVRKSQRSR QKKTT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6238 Collapse assay details

Data source: Panorama

Official Gene Symbol
CXCL9
Peptide Sequence
IEIIATLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
61
Peptide End
68
CPTAC ID
CPTAC-6238
Peptide Molecular Mass
899.5692
Species
Homo Sapiens
Assay Type
Enrichment MRM
Enrichment Method
Peptide Immunoaffinity
Matrix
Frozen tissue
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
Extended heavy peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent
Column Packing
Reprosil C18, 3um
Column Dimensions
75µm x 15cm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 11.2 11.4 7.6 10.3 13.2 15.3 15.2 17.4 17.1 15 15 15
y6 (1+) 18.6 9.7 6.5 9 11.1 16.2 20.7 14.7 17.5 15 15 15
y5 (1+) 11.9 10.9 6.8 9.4 10.3 14 15.2 15 15.6 15 15 15
sum 15.5 9 5.4 8.1 10 15.1 17.5 13.5 16 15 15 15


Additional Resources and Comments