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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ITGAM CD11B, CR3A
Sequence Length (AA) Molecular Weight (Da)
1152 127179
Protein Name
Integrin alpha-M
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALRVLLLTA LTLCHGFNLD TENAMTFQEN ARGFGQSVVQ LQGSRVVVGA
60 70 80 90 100
PQEIVAANQR GSLYQCDYST GSCEPIRLQV PVEAVNMSLG LSLAATTSPP
110 120 130 140 150
QLLACGPTVH QTCSENTYVK GLCFLFGSNL RQQPQKFPEA LRGCPQEDSD
160 170 180 190 200
IAFLIDGSGS IIPHDFRRMK EFVSTVMEQL KKSKTLFSLM QYSEEFRIHF
210 220 230 240 250
TFKEFQNNPN PRSLVKPITQ LLGRTHTATG IRKVVRELFN ITNGARKNAF
260 270 280 290 300
KILVVITDGE KFGDPLGYED VIPEADREGV IRYVIGVGDA FRSEKSRQEL
310 320 330 340 350
NTIASKPPRD HVFQVNNFEA LKTIQNQLRE KIFAIEGTQT GSSSSFEHEM
360 370 380 390 400
SQEGFSAAIT SNGPLLSTVG SYDWAGGVFL YTSKEKSTFI NMTRVDSDMN
410 420 430 440 450
DAYLGYAAAI ILRNRVQSLV LGAPRYQHIG LVAMFRQNTG MWESNANVKG
460 470 480 490 500
TQIGAYFGAS LCSVDVDSNG STDLVLIGAP HYYEQTRGGQ VSVCPLPRGR
510 520 530 540 550
ARWQCDAVLY GEQGQPWGRF GAALTVLGDV NGDKLTDVAI GAPGEEDNRG
560 570 580 590 600
AVYLFHGTSG SGISPSHSQR IAGSKLSPRL QYFGQSLSGG QDLTMDGLVD
610 620 630 640 650
LTVGAQGHVL LLRSQPVLRV KAIMEFNPRE VARNVFECND QVVKGKEAGE
660 670 680 690 700
VRVCLHVQKS TRDRLREGQI QSVVTYDLAL DSGRPHSRAV FNETKNSTRR
710 720 730 740 750
QTQVLGLTQT CETLKLQLPN CIEDPVSPIV LRLNFSLVGT PLSAFGNLRP
760 770 780 790 800
VLAEDAQRLF TALFPFEKNC GNDNICQDDL SITFSFMSLD CLVVGGPREF
810 820 830 840 850
NVTVTVRNDG EDSYRTQVTF FFPLDLSYRK VSTLQNQRSQ RSWRLACESA
860 870 880 890 900
SSTEVSGALK STSCSINHPI FPENSEVTFN ITFDVDSKAS LGNKLLLKAN
910 920 930 940 950
VTSENNMPRT NKTEFQLELP VKYAVYMVVT SHGVSTKYLN FTASENTSRV
960 970 980 990 1000
MQHQYQVSNL GQRSLPISLV FLVPVRLNQT VIWDRPQVTF SENLSSTCHT
1010 1020 1030 1040 1050
KERLPSHSDF LAELRKAPVV NCSIAVCQRI QCDIPFFGIQ EEFNATLKGN
1060 1070 1080 1090 1100
LSFDWYIKTS HNHLLIVSTA EILFNDSVFT LLPGQGAFVR SQTETKVEPF
1110 1120 1130 1140 1150
EVPNPLPLIV GSSVGGLLLL ALITAALYKL GFFKRQYKDM MSEGGPPGAE
1152
PQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6241 Collapse assay details

Data source: Panorama

Official Gene Symbol
ITGAM
Peptide Modified Sequence
LAC[+57.021464]ESASSTEVSGALK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
845
Peptide End
860
CPTAC ID
CPTAC-6241
Peptide Molecular Mass
1,608.7665
Species
Homo Sapiens
Assay Type
Enrichment MRM
Enrichment Method
Peptide Immunoaffinity
Matrix
Frozen tissue
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
Extended heavy peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent
Column Packing
Reprosil C18, 3um
Column Dimensions
75µm x 15cm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 8.7 7 3.1 14.3 4.2 4.7 16.7 8.2 5.6 15 15 15
y12 (1+) 9.5 5.9 3.1 9.4 4.9 4.3 13.4 7.7 5.3 15 15 15
y9 (1+) 8 7.2 3.9 12.8 4.6 5.7 15.1 8.5 6.9 15 15 15
y5 (1+) 10.3 6.4 3.2 12.4 5.3 5.2 16.1 8.3 6.1 15 15 15
sum 8.6 6.6 2.6 12.4 3.8 4.4 15.1 7.6 5.1 15 15 15


Additional Resources and Comments