IFNGR1, CPTAC-6243 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IFNGR1	N/A

Sequence Length (AA)	Molecular Weight (Da)
489	54405

Protein Name
Interferon gamma receptor 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MALLFLLPLV	MQGVSRAEMG	TADLGPSSVP	TPTNVTIESY	NMNPIVYWEY
60	70	80	90	100
QIMPQVPVFT	VEVKNYGVKN	SEWIDACINI	SHHYCNISDH	VGDPSNSLWV
110	120	130	140	150
RVKARVGQKE	SAYAKSEEFA	VCRDGKIGPP	KLDIRKEEKQ	IMIDIFHPSV
160	170	180	190	200
FVNGDEQEVD	YDPETTCYIR	VYNVYVRMNG	SEIQYKILTQ	KEDDCDEIQC
210	220	230	240	250
QLAIPVSSLN	SQYCVSAEGV	LHVWGVTTEK	SKEVCITIFN	SSIKGSLWIP
260	270	280	290	300
VVAALLLFLV	LSLVFICFYI	KKINPLKEKS	IILPKSLISV	VRSATLETKP
310	320	330	340	350
ESKYVSLITS	YQPFSLEKEV	VCEEPLSPAT	VPGMHTEDNP	GKVEHTEELS
360	370	380	390	400
SITEVVTTEE	NIPDVVPGSH	LTPIERESSS	PLSSNQSEPG	SIALNSYHSR
410	420	430	440	450
NCSESDHSRN	GFDTDSSCLE	SHSSLSDSEF	PPNNKGEIKT	EGQELITVIK
460	470	480	489
APTSFGYDKP	HVLVDLLVDD	SGKESLIGYR	PTEDSKEFS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6243 Collapse assay details

Data source: Panorama

Official Gene Symbol: IFNGR1
Peptide Sequence: YVSLITSYQPFSLEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 304
Peptide End: 318

CPTAC ID: CPTAC-6243
Peptide Molecular Mass: 1,773.9189
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	13.8	11.8	4.5	11.1	7.1	14.8	17.7	13.8	15.5	15	15	15
y10 (1+)	19.3	10.5	4	15.2	9.7	13.9	24.6	14.3	14.5	15	15	15
y8 (1+)	11.9	11.5	3.8	10.7	9.3	15	16	14.8	15.5	15	15	15
y6 (1+)	10.3	11.1	4.7	11.2	7.1	13.1	15.2	13.2	13.9	15	15	15
sum	13.8	10.4	3.3	9.8	7.1	13.7	16.9	12.6	14.1	15	15	15

IFNGR1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6243 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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IFNGR1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6243 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments