TSHB, CPTAC-691 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TSHB	N/A

Sequence Length (AA)	Molecular Weight (Da)
138	15639

Protein Name
Thyrotropin subunit beta

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTALFLMSML	FGLTCGQAMS	FCIPTEYTMH	IERRECAYCL	TINTTICAGY
60	70	80	90	100
CMTRDINGKL	FLPKYALSQD	VCTYRDFIYR	TVEIPGCPLH	VAPYFSYPVA
110	120	130	138
LSCKCGKCNT	DYSDCIHEAI	KTNYCTKPQK	SYLVGFSV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-691 Collapse assay details

Data source: Panorama

Official Gene Symbol: TSHB
Peptide Sequence: SYLVGFSV
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 131
Peptide End: 138

CPTAC ID: CPTAC-691
Peptide Molecular Mass: 870.4487
Species: Homo sapiens (Human)
Assay Type: Enrichment ImmunoMRM
Enrichment Method: peptide immunoaffinity
Matrix: plasma
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

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Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 Qtrap
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N

LC: Eksigent NanoLC 2D
Column Packing: ReproSil-Pur C18-AQ 3 um
Column Dimensions: 100 mm x 75 um ID
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7 (1+)	11.9	8.2	5.6	17.6	17	12.6	21.2	18.9	13.8	15	15	15
b6 (1+)	12.1	6	5.1	12.1	12.7	16.8	17.1	14	17.6	15	15	15
b3 (1+)	9.6	4.3	6.8	16.6	9.7	9.3	19.2	10.6	11.5	15	15	15
y5 (1+)	15	7.1	6.1	16.3	12.5	9.9	22.2	14.4	11.6	15	15	15
sum	6.9	3.2	3	10.9	11.4	11	12.9	11.8	11.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

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