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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TSHB N/A
Sequence Length (AA) Molecular Weight (Da)
138 15639
Protein Name
Thyrotropin subunit beta
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTALFLMSML FGLTCGQAMS FCIPTEYTMH IERRECAYCL TINTTICAGY
60 70 80 90 100
CMTRDINGKL FLPKYALSQD VCTYRDFIYR TVEIPGCPLH VAPYFSYPVA
110 120 130 138
LSCKCGKCNT DYSDCIHEAI KTNYCTKPQK SYLVGFSV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-691 Collapse assay details

Data source: Panorama

Official Gene Symbol
TSHB
Peptide Sequence
SYLVGFSV
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
131
Peptide End
138
CPTAC ID
CPTAC-691
Peptide Molecular Mass
870.4487
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 11.9 8.2 5.6 17.6 17 12.6 21.2 18.9 13.8 15 15 15
b6 (1+) 12.1 6 5.1 12.1 12.7 16.8 17.1 14 17.6 15 15 15
b3 (1+) 9.6 4.3 6.8 16.6 9.7 9.3 19.2 10.6 11.5 15 15 15
y5 (1+) 15 7.1 6.1 16.3 12.5 9.9 22.2 14.4 11.6 15 15 15
sum 6.9 3.2 3 10.9 11.4 11 12.9 11.8 11.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-729 Collapse assay details

Data source: Panorama

Official Gene Symbol
TSHB
Peptide Modified Sequence
YALSQDVC[+57.0]TYR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
72
Peptide - Site of Modification
8
Peptide Start
65
Peptide End
75
CPTAC ID
CPTAC-729
Peptide Molecular Mass
1,374.6238
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 23 4.6 3.6 25.8 7.8 8.4 34.6 9.1 9.1 15 15 15
y7 (1+) 10.8 4.4 5.4 11.4 9.5 9.6 15.7 10.5 11 15 15 15
y8 (1+) 9.3 2.9 2.4 12.8 7.8 6.9 15.8 8.3 7.3 15 15 15
y9 (1+) 11.3 3.9 2.7 10.6 5.9 4.8 15.5 7.1 5.5 15 15 15
sum 8.2 1.3 2.4 11.3 7 6.9 14 7.1 7.3 15 15 15


Additional Resources and Comments