CGB, CPTAC-718 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CGB	N/A

Sequence Length (AA)	Molecular Weight (Da)
165	17739

Protein Name
Choriogonadotropin subunit beta

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEMFQGLLLL	LLLSMGGTWA	SKEPLRPRCR	PINATLAVEK	EGCPVCITVN
60	70	80	90	100
TTICAGYCPT	MTRVLQGVLP	ALPQVVCNYR	DVRFESIRLP	GCPRGVNPVV
110	120	130	140	150
SYAVALSCQC	ALCRRSTTDC	GGPKDHPLTC	DDPRFQDSSS	SKAPPPSLPS
160	165
PSRLPGPSDT	PILPQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-718 Collapse assay details

Data source: Panorama

Official Gene Symbol: CGB
Peptide Modified Sequence: VLQGVLPALPQVVC[+57.0]NYR
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: 77
Peptide - Site of Modification: 14
Peptide Start: 64
Peptide End: 80

CPTAC ID: CPTAC-718
Peptide Molecular Mass: 1,925.0557
Species: Homo sapiens (Human)
Assay Type: Enrichment ImmunoMRM
Enrichment Method: peptide immunoaffinity
Matrix: plasma
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

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Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 Qtrap
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent NanoLC 2D
Column Packing: ReproSil-Pur C18-AQ 3 um
Column Dimensions: 100 mm x 75 um ID
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	36.5	14.9	14.6	55.5	31.9	33.2	66.4	35.2	36.3	15	15	15
y9 (1+)	34.2	19.8	17.9	39.5	27.8	27.5	52.2	34.1	32.8	15	15	15
y10 (1+)	43.8	23.4	18.4	59.9	35.4	29.5	74.2	42.4	34.8	15	15	15
sum	30.2	12	11.6	45.7	28.4	29.5	54.8	30.8	31.7	15	15	15

CGB

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-718 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CGB

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-718 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments