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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CGB N/A
Sequence Length (AA) Molecular Weight (Da)
165 17739
Protein Name
Choriogonadotropin subunit beta
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEMFQGLLLL LLLSMGGTWA SKEPLRPRCR PINATLAVEK EGCPVCITVN
60 70 80 90 100
TTICAGYCPT MTRVLQGVLP ALPQVVCNYR DVRFESIRLP GCPRGVNPVV
110 120 130 140 150
SYAVALSCQC ALCRRSTTDC GGPKDHPLTC DDPRFQDSSS SKAPPPSLPS
160 165
PSRLPGPSDT PILPQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-718 Collapse assay details

Data source: Panorama

Official Gene Symbol
CGB
Peptide Modified Sequence
VLQGVLPALPQVVC[+57.0]NYR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
77
Peptide - Site of Modification
14
Peptide Start
64
Peptide End
80
CPTAC ID
CPTAC-718
Peptide Molecular Mass
1,925.0557
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 36.5 14.9 14.6 55.5 31.9 33.2 66.4 35.2 36.3 15 15 15
y9 (1+) 34.2 19.8 17.9 39.5 27.8 27.5 52.2 34.1 32.8 15 15 15
y10 (1+) 43.8 23.4 18.4 59.9 35.4 29.5 74.2 42.4 34.8 15 15 15
sum 30.2 12 11.6 45.7 28.4 29.5 54.8 30.8 31.7 15 15 15

Additional Resources and Comments