SERPINA6, CPTAC-717 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SERPINA6	CBG

Sequence Length (AA)	Molecular Weight (Da)
405	45141

Protein Name
Corticosteroid-binding globulin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPLLLYTCLL	WLPTSGLWTV	QAMDPNAAYV	NMSNHHRGLA	SANVDFAFSL
60	70	80	90	100
YKHLVALSPK	KNIFISPVSI	SMALAMLSLG	TCGHTRAQLL	QGLGFNLTER
110	120	130	140	150
SETEIHQGFQ	HLHQLFAKSD	TSLEMTMGNA	LFLDGSLELL	ESFSADIKHY
160	170	180	190	200
YESEVLAMNF	QDWATASRQI	NSYVKNKTQG	KIVDLFSGLD	SPAILVLVNY
210	220	230	240	250
IFFKGTWTQP	FDLASTREEN	FYVDETTVVK	VPMMLQSSTI	SYLHDSELPC
260	270	280	290	300
QLVQMNYVGN	GTVFFILPDK	GKMNTVIAAL	SRDTINRWSA	GLTSSQVDLY
310	320	330	340	350
IPKVTISGVY	DLGDVLEEMG	IADLFTNQAN	FSRITQDAQL	KSSKVVHKAV
360	370	380	390	400
LQLNEEGVDT	AGSTGVTLNL	TSKPIILRFN	QPFIIMIFDH	FTWSSLFLAR
405
VMNPV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-717 Collapse assay details

Data source: Panorama

Official Gene Symbol: SERPINA6
Peptide Sequence: AQLLQGLGFNLTER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 87
Peptide End: 100

CPTAC ID: CPTAC-717
Peptide Molecular Mass: 1,558.8467
Species: Homo sapiens (Human)
Assay Type: Enrichment ImmunoMRM
Enrichment Method: peptide immunoaffinity
Matrix: plasma
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 Qtrap
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent NanoLC 2D
Column Packing: ReproSil-Pur C18-AQ 3 um
Column Dimensions: 100 mm x 75 um ID
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	33.7	12.2	17.7	51.8	24.1	29.7	61.8	27	34.6	15	15	15
y9 (1+)	33.8	13.6	18.8	40.8	27.2	31.5	53	30.4	36.7	15	15	15
y10 (1+)	26.4	18	22.6	36.1	23.1	44.4	44.7	29.3	49.8	15	15	15
sum	21.7	11.8	9.2	35.2	22	22.2	41.4	25	24	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b4 (2+)	69	59	51.7	63.7	75.2	61.1	93.9	95.6	80	15	15	15
b2 (1+)	6	5.9	3.4	7	7.2	5.5	9.2	9.3	6.5	15	15	15
y9 (3+)	8	4.7	4	13.4	6.6	5.4	15.6	8.1	6.7	15	15	15
y7 (2+)	7.3	4.5	2.3	8.8	7.5	3.6	11.4	8.7	4.3	15	15	15
y9 (2+)	8.2	3.3	6.3	9.8	7.5	5.9	12.8	8.2	8.6	15	15	15
sum	4.9	1.7	4	7.3	6.2	3.9	8.8	6.4	5.6	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	31.8	10.8	5.9	35.8	12.3	8.9	47.9	16.4	10.7	15	15	15
b4 (1+)	10.5	10.8	8.7	22.1	11.2	8.1	24.5	15.6	11.9	15	15	15
y6 (1+)	12.8	6.8	5	16.6	8.6	8.3	21	11	9.7	15	15	15
y3 (1+)	13	9.1	5.5	13.7	13.8	6.4	18.9	16.5	8.4	15	15	15
y7 (1+)	16.6	9.9	8.6	17.7	9.9	9.2	24.3	14	12.6	15	15	15
sum	7.1	4.2	3.7	9.2	7.3	5.4	11.6	8.4	6.5	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7 (2+)	12.3	5.6	10.4	13.4	10.7	14.3	18.2	12.1	17.7	15	15	15
b2 (1+)	8	12.4	11	10.6	16.4	9.7	13.3	20.6	14.7	15	15	15
y2 (1+)	9.7	12.2	11.5	12.6	12.8	11.9	15.9	17.7	16.5	15	15	15
y3 (1+)	12	11.3	13.1	14.4	11.4	14.3	18.7	16.1	19.4	15	15	15
y5 (1+)	13.4	10.2	7.3	18.8	11.7	8.2	23.1	15.5	11	15	15	15
sum	4	4.5	4.9	12.1	6.9	5.4	12.7	8.2	7.3	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	13.5	11.5	6.9	16.9	11	8.2	21.6	15.9	10.7	15	15	15
y14 (2+)	20.5	10.8	11.3	25.2	12.8	13.8	32.5	16.7	17.8	15	15	15
b5 (1+)	22	9.4	8.8	19.2	12.3	11.1	29.2	15.5	14.2	15	15	15
y4 (1+)	29.7	15.6	13.6	46.9	15.9	15.4	55.5	22.3	20.5	15	15	15
y2 (1+)	10.7	7.3	7.1	13.2	10.3	8.3	17	12.6	10.9	15	15	15
sum	9.9	5.4	6.8	13.3	8.8	7.9	16.6	10.3	10.4	15	15	15

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SERPINA6

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-717 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Assay Details for non-CPTAC-2661 Collapse assay details

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Assay Details for non-CPTAC-3374 Collapse assay details

Assay Parameters Collapse assay parameters

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Repeatability

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Assay Details for non-CPTAC-2662 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

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