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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPINA6 CBG
Sequence Length (AA) Molecular Weight (Da)
405 45141
Protein Name
Corticosteroid-binding globulin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPLLLYTCLL WLPTSGLWTV QAMDPNAAYV NMSNHHRGLA SANVDFAFSL
60 70 80 90 100
YKHLVALSPK KNIFISPVSI SMALAMLSLG TCGHTRAQLL QGLGFNLTER
110 120 130 140 150
SETEIHQGFQ HLHQLFAKSD TSLEMTMGNA LFLDGSLELL ESFSADIKHY
160 170 180 190 200
YESEVLAMNF QDWATASRQI NSYVKNKTQG KIVDLFSGLD SPAILVLVNY
210 220 230 240 250
IFFKGTWTQP FDLASTREEN FYVDETTVVK VPMMLQSSTI SYLHDSELPC
260 270 280 290 300
QLVQMNYVGN GTVFFILPDK GKMNTVIAAL SRDTINRWSA GLTSSQVDLY
310 320 330 340 350
IPKVTISGVY DLGDVLEEMG IADLFTNQAN FSRITQDAQL KSSKVVHKAV
360 370 380 390 400
LQLNEEGVDT AGSTGVTLNL TSKPIILRFN QPFIIMIFDH FTWSSLFLAR
405
VMNPV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-717 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINA6
Peptide Sequence
AQLLQGLGFNLTER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
87
Peptide End
100
CPTAC ID
CPTAC-717
Peptide Molecular Mass
1,558.8467
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 33.7 12.2 17.7 51.8 24.1 29.7 61.8 27 34.6 15 15 15
y9 (1+) 33.8 13.6 18.8 40.8 27.2 31.5 53 30.4 36.7 15 15 15
y10 (1+) 26.4 18 22.6 36.1 23.1 44.4 44.7 29.3 49.8 15 15 15
sum 21.7 11.8 9.2 35.2 22 22.2 41.4 25 24 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2661 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINA6
Peptide Sequence
HLVALSPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
53
Peptide End
60
CPTAC ID
non-CPTAC-2661
Peptide Molecular Mass
863.5229
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 31.8 10.8 5.9 35.8 12.3 8.9 47.9 16.4 10.7 15 15 15
b4 (1+) 10.5 10.8 8.7 22.1 11.2 8.1 24.5 15.6 11.9 15 15 15
y6 (1+) 12.8 6.8 5 16.6 8.6 8.3 21 11 9.7 15 15 15
y3 (1+) 13 9.1 5.5 13.7 13.8 6.4 18.9 16.5 8.4 15 15 15
y7 (1+) 16.6 9.9 8.6 17.7 9.9 9.2 24.3 14 12.6 15 15 15
sum 7.1 4.2 3.7 9.2 7.3 5.4 11.6 8.4 6.5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2662 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINA6
Peptide Sequence
WSAGLTSSQVDLYIPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
288
Peptide End
303
CPTAC ID
non-CPTAC-2662
Peptide Molecular Mass
1,763.9094
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 13.5 11.5 6.9 16.9 11 8.2 21.6 15.9 10.7 15 15 15
y14 (2+) 20.5 10.8 11.3 25.2 12.8 13.8 32.5 16.7 17.8 15 15 15
b5 (1+) 22 9.4 8.8 19.2 12.3 11.1 29.2 15.5 14.2 15 15 15
y4 (1+) 29.7 15.6 13.6 46.9 15.9 15.4 55.5 22.3 20.5 15 15 15
y2 (1+) 10.7 7.3 7.1 13.2 10.3 8.3 17 12.6 10.9 15 15 15
sum 9.9 5.4 6.8 13.3 8.8 7.9 16.6 10.3 10.4 15 15 15

Additional Resources and Comments