BIRC2, CPTAC-791 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BIRC2	API1, IAP2, MIHB, RNF48

Sequence Length (AA)	Molecular Weight (Da)
618	69900

Protein Name
Baculoviral IAP repeat-containing protein 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MHKTASQRLF	PGPSYQNIKS	IMEDSTILSD	WTNSNKQKMK	YDFSCELYRM
60	70	80	90	100
STYSTFPAGV	PVSERSLARA	GFYYTGVNDK	VKCFCCGLML	DNWKLGDSPI
110	120	130	140	150
QKHKQLYPSC	SFIQNLVSAS	LGSTSKNTSP	MRNSFAHSLS	PTLEHSSLFS
160	170	180	190	200
GSYSSLSPNP	LNSRAVEDIS	SSRTNPYSYA	MSTEEARFLT	YHMWPLTFLS
210	220	230	240	250
PSELARAGFY	YIGPGDRVAC	FACGGKLSNW	EPKDDAMSEH	RRHFPNCPFL
260	270	280	290	300
ENSLETLRFS	ISNLSMQTHA	ARMRTFMYWP	SSVPVQPEQL	ASAGFYYVGR
310	320	330	340	350
NDDVKCFCCD	GGLRCWESGD	DPWVEHAKWF	PRCEFLIRMK	GQEFVDEIQG
360	370	380	390	400
RYPHLLEQLL	STSDTTGEEN	ADPPIIHFGP	GESSSEDAVM	MNTPVVKSAL
410	420	430	440	450
EMGFNRDLVK	QTVQSKILTT	GENYKTVNDI	VSALLNAEDE	KREEEKEKQA
460	470	480	490	500
EEMASDDLSL	IRKNRMALFQ	QLTCVLPILD	NLLKANVINK	QEHDIIKQKT
510	520	530	540	550
QIPLQARELI	DTILVKGNAA	ANIFKNCLKE	IDSTLYKNLF	VDKNMKYIPT
560	570	580	590	600
EDVSGLSLEE	QLRRLQEERT	CKVCMDKEVS	VVFIPCGHLV	VCQECAPSLR
610	618
KCPICRGIIK	GTVRTFLS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-790 Collapse assay details

Data source: Panorama

Official Gene Symbol: BIRC2
Peptide Sequence: AVEDISSSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 165
Peptide End: 173

CPTAC ID: CPTAC-790
Peptide Molecular Mass: 962.4669
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	11.4	6.1	5	11.2	3.9	5	16	7.2	7.1	15	15	15
y5 (1+)	8	6.5	3.7	9.7	6.4	4	12.6	9.1	5.4	15	15	15
y6 (1+)	7.6	8.9	3.2	7.2	8.8	3.6	10.5	12.5	4.8	15	15	15
y7 (1+)	6.2	5	5.2	7.1	4.5	7	9.4	6.7	8.7	15	15	15
sum	4.2	4.1	2.5	3.3	4	3.1	5.3	5.7	4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-791 Collapse assay details

Data source: Panorama

Official Gene Symbol: BIRC2
Peptide Sequence: LGDSPIQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 95
Peptide End: 102

CPTAC ID: CPTAC-791
Peptide Molecular Mass: 856.4654
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend