MAP2K3, CPTAC-815 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAP2K3	MEK3, MKK3, PRKMK3, SKK2

Sequence Length (AA)	Molecular Weight (Da)
347	39318

Protein Name
Dual specificity mitogen-activated protein kinase kinase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MESPASSQPA	SMPQSKGKSK	RKKDLRISCM	SKPPAPNPTP	PRNLDSRTFI
60	70	80	90	100
TIGDRNFEVE	ADDLVTISEL	GRGAYGVVEK	VRHAQSGTIM	AVKRIRATVN
110	120	130	140	150
SQEQKRLLMD	LDINMRTVDC	FYTVTFYGAL	FREGDVWICM	ELMDTSLDKF
160	170	180	190	200
YRKVLDKNMT	IPEDILGEIA	VSIVRALEHL	HSKLSVIHRD	VKPSNVLINK
210	220	230	240	250
EGHVKMCDFG	ISGYLVDSVA	KTMDAGCKPY	MAPERINPEL	NQKGYNVKSD
260	270	280	290	300
VWSLGITMIE	MAILRFPYES	WGTPFQQLKQ	VVEEPSPQLP	ADRFSPEFVD
310	320	330	340	347
FTAQCLRKNP	AERMSYLELM	EHPFFTLHKT	KKTDIAAFVK	EILGEDS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-814 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP2K3
Peptide Modified Sequence: FSPEFVDFTAQC[+57.0]LR
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: 305
Peptide - Site of Modification: 12
Peptide Start: 294
Peptide End: 307

CPTAC ID: CPTAC-814
Peptide Molecular Mass: 1,715.7977
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	40.5	20.7	15.9	41.9	21.3	15.3	58.3	29.7	22.1	15	15	15
y6 (1+)	22.7	14.5	6	21.4	16.9	10.4	31.2	22.3	12	15	15	15
y7 (1+)	14.8	12.1	7.1	16.2	13	6.8	21.9	17.8	9.8	15	15	15
y8 (1+)	15.8	12.5	8.4	15.4	11.7	8.3	22.1	17.1	11.8	15	15	15
y9 (1+)	10.3	8.9	6.7	13.4	8.8	7.5	16.9	12.5	10.1	15	15	15
y10 (1+)	21.5	8	6.2	25.3	13.8	6.7	33.2	16	9.1	15	15	15
y11 (1+)	27.8	22	15.5	35.8	26	20	45.3	34.1	25.3	15	15	15
sum	7.5	5	3.1	7	6.7	3.1	10.3	8.4	4.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-815 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP2K3
Peptide Sequence: QVVEEPSPQLPADR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 280
Peptide End: 293

CPTAC ID: CPTAC-815
Peptide Molecular Mass: 1,563.7893
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend