MAP2K4, CPTAC-816 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAP2K4	JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1

Sequence Length (AA)	Molecular Weight (Da)
399	44288

Protein Name
Dual specificity mitogen-activated protein kinase kinase 4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAPSPSGGG	GSGGGSGSGT	PGPVGSPAPG	HPAVSSMQGK	RKALKLNFAN
60	70	80	90	100
PPFKSTARFT	LNPNPTGVQN	PHIERLRTHS	IESSGKLKIS	PEQHWDFTAE
110	120	130	140	150
DLKDLGEIGR	GAYGSVNKMV	HKPSGQIMAV	KRIRSTVDEK	EQKQLLMDLD
160	170	180	190	200
VVMRSSDCPY	IVQFYGALFR	EGDCWICMEL	MSTSFDKFYK	YVYSVLDDVI
210	220	230	240	250
PEEILGKITL	ATVKALNHLK	ENLKIIHRDI	KPSNILLDRS	GNIKLCDFGI
260	270	280	290	300
SGQLVDSIAK	TRDAGCRPYM	APERIDPSAS	RQGYDVRSDV	WSLGITLYEL
310	320	330	340	350
ATGRFPYPKW	NSVFDQLTQV	VKGDPPQLSN	SEEREFSPSF	INFVNLCLTK
360	370	380	390	399
DESKRPKYKE	LLKHPFILMY	EERAVEVACY	VCKILDQMPA	TPSSPMYVD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-816 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP2K4
Peptide Modified Sequence: AVEVAC[+57.0]YVC[+57.0]K
Modification Type: Carbamidomethyl Cysteine, Carbamidomethyl Cysteine
Protein - Site of Modification: 379, 382
Peptide - Site of Modification: 6, 9
Peptide Start: 374
Peptide End: 383

CPTAC ID: CPTAC-816
Peptide Molecular Mass: 1,197.5522
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	9.7	9.8	9	10.2	7.2	8.1	14.1	12.2	12.1	15	15	15
y4 (1+)	13.1	6.5	8.3	12.2	9.5	7.7	17.9	11.5	11.3	15	15	15
y5 (1+)	12	9	8.2	11	9.9	7.4	16.3	13.4	11	15	15	15
y6 (1+)	15.6	4.7	6.3	17.3	5.1	6.8	23.3	6.9	9.3	15	15	15
y7 (1+)	10.5	7.5	8.9	9.8	10.4	10.8	14.4	12.8	14	15	15	15
y8 (1+)	8.7	4.6	7.7	10.9	7.1	6.6	13.9	8.5	10.1	15	15	15
b3 (1+)	10.5	8.3	5.5	12.1	10.3	5.6	16	13.2	7.8	15	15	15
sum	4.9	2.7	3.5	5	2.6	3.8	7	3.7	5.2	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-817 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP2K4
Peptide Sequence: FTLNPNPTGVQNPHIER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 59
Peptide End: 75

CPTAC ID: CPTAC-817
Peptide Molecular Mass: 1,932.9806
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend