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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP2K4 JNKK1,MEK4,MKK4,PRKMK4,SEK1,SERK1,SKK1
Sequence Length (AA) Molecular Weight (Da)
399 44288
Protein Name
Dual specificity mitogen-activated protein kinase kinase 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAPSPSGGG GSGGGSGSGT PGPVGSPAPG HPAVSSMQGK RKALKLNFAN
60 70 80 90 100
PPFKSTARFT LNPNPTGVQN PHIERLRTHS IESSGKLKIS PEQHWDFTAE
110 120 130 140 150
DLKDLGEIGR GAYGSVNKMV HKPSGQIMAV KRIRSTVDEK EQKQLLMDLD
160 170 180 190 200
VVMRSSDCPY IVQFYGALFR EGDCWICMEL MSTSFDKFYK YVYSVLDDVI
210 220 230 240 250
PEEILGKITL ATVKALNHLK ENLKIIHRDI KPSNILLDRS GNIKLCDFGI
260 270 280 290 300
SGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV WSLGITLYEL
310 320 330 340 350
ATGRFPYPKW NSVFDQLTQV VKGDPPQLSN SEEREFSPSF INFVNLCLTK
360 370 380 390 399
DESKRPKYKE LLKHPFILMY EERAVEVACY VCKILDQMPA TPSSPMYVD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-816 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K4
Peptide Modified Sequence
AVEVAC[+57.0]YVC[+57.0]K
Modification Type
Carbamidomethyl Cysteine, Carbamidomethyl Cysteine
Protein - Site of Modification
379, 382
Peptide - Site of Modification
6, 9
Peptide Start
374
Peptide End
383
CPTAC ID
CPTAC-816
Peptide Molecular Mass
1,197.5522
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.7 9.8 9 10.2 7.2 8.1 14.1 12.2 12.1 15 15 15
y4 (1+) 13.1 6.5 8.3 12.2 9.5 7.7 17.9 11.5 11.3 15 15 15
y5 (1+) 12 9 8.2 11 9.9 7.4 16.3 13.4 11 15 15 15
y6 (1+) 15.6 4.7 6.3 17.3 5.1 6.8 23.3 6.9 9.3 15 15 15
y7 (1+) 10.5 7.5 8.9 9.8 10.4 10.8 14.4 12.8 14 15 15 15
y8 (1+) 8.7 4.6 7.7 10.9 7.1 6.6 13.9 8.5 10.1 15 15 15
b3 (1+) 10.5 8.3 5.5 12.1 10.3 5.6 16 13.2 7.8 15 15 15
sum 4.9 2.7 3.5 5 2.6 3.8 7 3.7 5.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-817 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K4
Peptide Sequence
FTLNPNPTGVQNPHIER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
59
Peptide End
75
CPTAC ID
CPTAC-817
Peptide Molecular Mass
1,932.9806
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 16.5 5.3 4.5 20.4 8 6.6 26.2 9.6 8 15 15 15
y4 (1+) 13.3 12.5 8.2 27.6 12.5 8.9 30.6 17.7 12.1 15 15 15
y5 (1+) 6.3 2.2 5.1 7 3.4 5.9 9.4 4 7.8 15 15 15
y6 (1+) 14.5 7.9 5.6 12.5 10 4.5 19.1 12.7 7.2 15 15 15
y7 (1+) 8.4 4.7 8.4 8.1 7.4 8.1 11.7 8.8 11.7 15 15 15
y8 (1+) 20.5 11.1 8.1 15.6 12.1 10.3 25.8 16.4 13.1 15 15 15
y9 (1+) 13.6 6.7 3.9 16 7 4.9 21 9.7 6.3 15 15 15
y10 (1+) 12 7.3 5.8 13.2 7.2 6.2 17.8 10.3 8.5 15 15 15
y11 (1+) 12.8 4.9 6 14.1 6.7 6.6 19 8.3 8.9 15 15 15
sum 5.1 1.8 2.1 5 2.1 3.2 7.1 2.8 3.8 15 15 15

Additional Resources and Comments