Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K11 MLK3, PTK1, SPRK
Sequence Length (AA) Molecular Weight (Da)
847 92688
Protein Name
Mitogen-activated protein kinase kinase kinase 11
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEPLKSLFLK SPLGSWNGSG SGGGGGGGGG RPEGSPKAAG YANPVWTALF
60 70 80 90 100
DYEPSGQDEL ALRKGDRVEV LSRDAAISGD EGWWAGQVGG QVGIFPSNYV
110 120 130 140 150
SRGGGPPPCE VASFQELRLE EVIGIGGFGK VYRGSWRGEL VAVKAARQDP
160 170 180 190 200
DEDISVTAES VRQEARLFAM LAHPNIIALK AVCLEEPNLC LVMEYAAGGP
210 220 230 240 250
LSRALAGRRV PPHVLVNWAV QIARGMHYLH CEALVPVIHR DLKSNNILLL
260 270 280 290 300
QPIESDDMEH KTLKITDFGL AREWHKTTQM SAAGTYAWMA PEVIKASTFS
310 320 330 340 350
KGSDVWSFGV LLWELLTGEV PYRGIDCLAV AYGVAVNKLT LPIPSTCPEP
360 370 380 390 400
FAQLMADCWA QDPHRRPDFA SILQQLEALE AQVLREMPRD SFHSMQEGWK
410 420 430 440 450
REIQGLFDEL RAKEKELLSR EEELTRAARE QRSQAEQLRR REHLLAQWEL
460 470 480 490 500
EVFERELTLL LQQVDRERPH VRRRRGTFKR SKLRARDGGE RISMPLDFKH
510 520 530 540 550
RITVQASPGL DRRRNVFEVG PGDSPTFPRF RAIQLEPAEP GQAWGRQSPR
560 570 580 590 600
RLEDSSNGER RACWAWGPSS PKPGEAQNGR RRSRMDEATW YLDSDDSSPL
610 620 630 640 650
GSPSTPPALN GNPPRPSLEP EEPKRPVPAE RGSSSGTPKL IQRALLRGTA
660 670 680 690 700
LLASLGLGRD LQPPGGPGRE RGESPTTPPT PTPAPCPTEP PPSPLICFSL
710 720 730 740 750
KTPDSPPTPA PLLLDLGIPV GQRSAKSPRR EEEPRGGTVS PPPGTSRSAP
760 770 780 790 800
GTPGTPRSPP LGLISRPRPS PLRSRIDPWS FVSAGPRPSP LPSPQPAPRR
810 820 830 840 847
APWTLFPDSD PFWDSPPANP FQGGPQDCRA QTKDMGAQAP WVPEAGP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-833 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K11
Peptide Sequence
AIQLEPAEPGQAWGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
532
Peptide End
546
CPTAC ID
CPTAC-833
Peptide Molecular Mass
1,621.8213
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 7 4.2 6.5 7 6.6 5.5 9.9 7.8 8.5 15 15 15
y10 (1+) 5 3.4 3.9 8.7 4.2 3.8 10 5.4 5.4 15 15 15
y11 (1+) 14.5 6.4 6.4 17.5 14 6.6 22.7 15.4 9.2 15 15 15
y12 (1+) 13.8 13.4 9 9.2 14.8 9.1 16.6 20 12.8 15 15 15
b3 (1+) 10.4 6.8 8.8 10.7 7.1 9.5 14.9 9.8 12.9 15 15 15
sum 4.7 3.5 2.9 5.6 4.6 2.9 7.3 5.8 4.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-835 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K11
Peptide Sequence
GGTVSPPPGTSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
736
Peptide End
747
CPTAC ID
CPTAC-835
Peptide Molecular Mass
1,111.5622
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 6 3.6 5.9 10.4 3.7 5.9 12 5.2 8.3 15 15 15
y6 (1+) 4.6 2.6 4.6 9.4 5.5 5.3 10.5 6.1 7 15 15 15
y7 (1+) 8.6 3.3 3.9 8.4 4.9 4.8 12 5.9 6.2 15 15 15
y8 (1+) 6.1 2.5 3.4 8.7 4.1 3.7 10.6 4.8 5 15 15 15
b3 (1+) 14.5 8.8 9.4 19.4 7.8 11.7 24.2 11.8 15 15 15 15
sum 4.7 1.8 3.2 6.8 3.8 3.9 8.3 4.2 5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1047 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K11
Peptide Modified Sequence
GGTVS[+80.0]PPPGTSR
Modification Type
Phospho (ST)
Protein - Site of Modification
740
Peptide - Site of Modification
5
Peptide Start
736
Peptide End
747
CPTAC ID
CPTAC-1047
Peptide Molecular Mass
1,191.5285
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 34.2 43.6 28.3 32.8 48.9 31.7 47.4 65.5 42.5 15 15 15
y7 (1+) 15.4 12.3 11.7 15.1 12.5 11 21.6 17.5 16.1 15 15 15
y6 (1+) 21.7 9.2 13.9 23.5 8.5 12.4 32 12.5 18.6 15 15 15
sum 12.7 6.6 8.8 13.8 6.2 8.2 18.8 9.1 12 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-836 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K11
Peptide Modified Sequence
ITVQAS[+80.0]PGLDR
Modification Type
Phospho (ST)
Protein - Site of Modification
507
Peptide - Site of Modification
6
Peptide Start
502
Peptide End
512
CPTAC ID
CPTAC-836
Peptide Molecular Mass
1,235.5911
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 14.1 10.7 6.6 13.9 9.4 7.5 19.8 14.2 10 15 15 15
y5 (1+) 8.5 3.5 3.5 8.7 3.8 3.8 12.2 5.2 5.2 15 15 15
y6 (1+) 22.2 14.8 14.1 23.1 15.8 15.8 32 21.6 21.2 15 15 15
y7 (1+) 15.7 11.7 10.9 15.5 12.9 13.2 22.1 17.4 17.1 15 15 15
y9 (1+) 31 11.1 16.1 30.7 12.7 17.4 43.6 16.9 23.7 15 15 15
sum 8.8 4.2 2.9 8.3 4.2 3 12.1 5.9 4.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-837 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K11
Peptide Sequence
ITVQASPGLDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
502
Peptide End
512
CPTAC ID
CPTAC-837
Peptide Molecular Mass
1,155.6248
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 10.4 4.6 9.4 10.7 7.1 8 14.9 8.5 12.3 15 15 15
y5 (1+) 7.8 2.7 4.4 7.2 5.6 4.6 10.6 6.2 6.4 15 15 15
y6 (1+) 8.6 4.3 4.3 8.3 5 5.8 12 6.6 7.2 15 15 15
y7 (1+) 3.6 4.7 2.9 5.3 4.3 3.9 6.4 6.4 4.9 15 15 15
y8 (1+) 5.3 3.9 3.1 6.8 5 3.2 8.6 6.3 4.5 15 15 15
y9 (1+) 6.5 3.9 3.7 4.9 3.7 4.5 8.1 5.4 5.8 15 15 15
y10 (1+) 10.4 8.5 5.6 12.7 10.5 5.9 16.4 13.5 8.1 15 15 15
sum 3.3 1.8 2 4.9 2.5 2.9 5.9 3.1 3.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-838 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K11
Peptide Sequence
LEEVIGIGGFGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
119
Peptide End
130
CPTAC ID
CPTAC-838
Peptide Molecular Mass
1,217.6656
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 8.7 7.2 6.2 9.9 7.6 7.2 13.2 10.5 9.5 15 15 15
y4 (1+) 6 8.8 5.9 6.5 10.2 7 8.8 13.5 9.2 15 15 15
y5 (1+) 7.2 3.6 3.2 7.9 3.5 3.7 10.7 5 4.9 15 15 15
y6 (1+) 11.7 6.3 7 15.4 6.3 7.3 19.3 8.9 10.1 15 15 15
y7 (1+) 8 3.4 1.9 7.8 3.8 2.6 11.2 5.1 3.2 15 15 15
y8 (1+) 5.1 3.4 2.3 6.5 4.1 4.4 8.3 5.3 5 15 15 15
y9 (1+) 11.6 7.9 7 9 9.4 5.8 14.7 12.3 9.1 15 15 15
y10 (1+) 9.6 7.8 6 8.1 9.2 7.7 12.6 12.1 9.8 15 15 15
b4 (1+) 19.9 11 6.2 20.1 11 5.7 28.3 15.6 8.4 15 15 15
b3 (1+) 5.8 3.6 4.6 4.6 4.2 5.4 7.4 5.5 7.1 15 15 15
sum 3.3 1.9 1.9 4 2.7 2.5 5.2 3.3 3.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-839 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K11
Peptide Modified Sequence
NVFEVGPGDS[+80.0]PTFPR
Modification Type
Phospho (ST)
Protein - Site of Modification
524
Peptide - Site of Modification
10
Peptide Start
515
Peptide End
529
CPTAC ID
CPTAC-839
Peptide Molecular Mass
1,697.7451
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 40.3 10.5 8.6 40.1 14.1 10.4 56.9 17.6 13.5 15 15 15
y5 (1+) 6.9 7.6 4.6 8.7 6.6 6.1 11.1 10.1 7.6 15 15 15
y6 (1+) 28 11.9 10.1 27.6 13.3 10.6 39.3 17.8 14.6 15 15 15
y10 (1+) 25.3 12.4 8.8 24.4 13.4 7.6 35.1 18.3 11.6 15 15 15
b3 (1+) 21.7 11.1 5.9 18.6 9.3 5.8 28.6 14.5 8.3 15 15 15
sum 7.3 4.7 3.7 7 5 4.5 10.1 6.9 5.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-840 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K11
Peptide Sequence
NVFEVGPGDSPTFPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
515
Peptide End
529
CPTAC ID
CPTAC-840
Peptide Molecular Mass
1,617.7787
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 14.8 6.2 4.5 12.6 5.9 3.8 19.4 8.6 5.9 15 15 15
y6 (1+) 3.4 3 3.1 5 3.5 3.1 6 4.6 4.4 15 15 15
y9 (1+) 5.1 4.3 3.1 6.5 4.9 4.3 8.3 6.5 5.3 15 15 15
y10 (1+) 5 3.4 1.8 4.3 3.9 1.9 6.6 5.2 2.6 15 15 15
y11 (1+) 8.8 4.2 3 9.6 5.5 3.4 13 6.9 4.5 15 15 15
y12 (1+) 13.4 8.8 5.3 10.4 9 4.9 17 12.6 7.2 15 15 15
b4 (1+) 13.2 6.8 7.2 13.7 8 8.3 19 10.5 11 15 15 15
b3 (1+) 12 4.1 4.3 14.4 8.4 5.5 18.7 9.3 7 15 15 15
sum 2.9 1.6 1.3 3.3 2.2 1.3 4.4 2.7 1.8 15 15 15


Additional Resources and Comments