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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAP3K2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K2 MAPKKK2, MEKK2
Sequence Length (AA) Molecular Weight (Da)
619 69741
Protein Name
Mitogen-activated protein kinase kinase kinase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDDQQALNSI MQDLAVLHKA SRPALSLQET RKAKSSSPKK QNDVRVKFEH
60 70 80 90 100
RGEKRILQFP RPVKLEDLRS KAKIAFGQSM DLHYTNNELV IPLTTQDDLD
110 120 130 140 150
KAVELLDRSI HMKSLKILLV INGSTQATNL EPLPSLEDLD NTVFGAERKK
160 170 180 190 200
RLSIIGPTSR DRSSPPPGYI PDELHQVARN GSFTSINSEG EFIPESMDQM
210 220 230 240 250
LDPLSLSSPE NSGSGSCPSL DSPLDGESYP KSRMPRAQSY PDNHQEFSDY
260 270 280 290 300
DNPIFEKFGK GGTYPRRYHV SYHHQEYNDG RKTFPRARRT QGTSLRSPVS
310 320 330 340 350
FSPTDHSLST SSGSSIFTPE YDDSRIRRRG SDIDNPTLTV MDISPPSRSP
360 370 380 390 400
RAPTNWRLGK LLGQGAFGRV YLCYDVDTGR ELAVKQVQFD PDSPETSKEV
410 420 430 440 450
NALECEIQLL KNLLHERIVQ YYGCLRDPQE KTLSIFMEYM PGGSIKDQLK
460 470 480 490 500
AYGALTENVT RKYTRQILEG VHYLHSNMIV HRDIKGANIL RDSTGNVKLG
510 520 530 540 550
DFGASKRLQT ICLSGTGMKS VTGTPYWMSP EVISGEGYGR KADIWSVACT
560 570 580 590 600
VVEMLTEKPP WAEFEAMAAI FKIATQPTNP KLPPHVSDYT RDFLKRIFVE
610 619
AKLRPSADEL LRHMFVHYH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-843 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K2
Peptide Sequence
AQSYPDNHQEFSDYDNPIFEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
237
Peptide End
257
CPTAC ID
CPTAC-843
Peptide Molecular Mass
2,543.0877
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 16 10.3 7.7 16.5 8.4 8.5 23 13.3 11.5 15 15 15
y5 (1+) 14.2 5.8 7.6 22.5 6.9 5.3 26.6 9 9.3 15 15 15
y6 (1+) 24 7.8 14.6 29 8.6 14.2 37.6 11.6 20.4 15 15 15
y7 (1+) 26.3 17.5 16.5 33.1 16.2 21.2 42.3 23.8 26.9 15 15 15
y9 (1+) 24 9.9 20.7 27.6 16.4 22.3 36.6 19.2 30.4 15 15 15
y11 (1+) 28.1 8.8 13.2 33.2 19.1 13 43.5 21 18.5 15 15 15
sum 9.6 4.7 5.1 11.1 4.3 3.2 14.7 6.4 6 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-2983 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K2
Peptide Sequence
AQSYPDNHQEFSDYDNPIFEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
237
Peptide End
257
CPTAC ID
CPTAC-2983
Peptide Molecular Mass
2,543.0877
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 9 5.1 18 10.2 11.7 24 13.6 12.8 30 15 15 15
b3 (1+) 9.1 5.4 4.3 8.9 5.2 4.1 12.7 7.5 5.9 15 15 15
y3 (1+) 8.9 5.4 2.3 7.2 6 2.5 11.4 8.1 3.4 15 15 15
sum 7 4.7 6.3 6.4 6.4 7.4 9.5 7.9 9.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-844 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K2
Peptide Sequence
LSIIGPTSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
152
Peptide End
160
CPTAC ID
CPTAC-844
Peptide Molecular Mass
942.5498
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 7.8 4.4 4.1 7.2 5.5 4.1 10.6 7 5.8 15 15 15
y5 (1+) 2.7 2.1 2.4 3 1.9 2.9 4 2.8 3.8 15 15 15
y6 (1+) 2.2 2.2 2 3.2 2.5 2.9 3.9 3.3 3.5 15 15 15
y7 (1+) 10.1 2.5 2.7 8.4 3.1 3.2 13.1 4 4.2 15 15 15
y8 (1+) 4.3 4.5 2.4 8.2 3.6 3.3 9.3 5.8 4.1 15 15 15
sum 1.5 1.7 1.7 2.4 1.8 2.4 2.8 2.5 2.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments