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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAP3K5

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K5 ASK1, MAPKKK5, MEKK5
Sequence Length (AA) Molecular Weight (Da)
1374 154537
Protein Name
Mitogen-activated protein kinase kinase kinase 5
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSTEADEGIT FSVPPFAPSG FCTIPEGGIC RRGGAAAVGE GEEHQLPPPP
60 70 80 90 100
PGSFWNVESA AAPGIGCPAA TSSSSATRGR GSSVGGGSRR TTVAYVINEA
110 120 130 140 150
SQGQLVVAES EALQSLREAC ETVGATLETL HFGKLDFGET TVLDRFYNAD
160 170 180 190 200
IAVVEMSDAF RQPSLFYHLG VRESFSMANN IILYCDTNSD SLQSLKEIIC
210 220 230 240 250
QKNTMCTGNY TFVPYMITPH NKVYCCDSSF MKGLTELMQP NFELLLGPIC
260 270 280 290 300
LPLVDRFIQL LKVAQASSSQ YFRESILNDI RKARNLYTGK ELAAELARIR
310 320 330 340 350
QRVDNIEVLT ADIVINLLLS YRDIQDYDSI VKLVETLEKL PTFDLASHHH
360 370 380 390 400
VKFHYAFALN RRNLPGDRAK ALDIMIPMVQ SEGQVASDMY CLVGRIYKDM
410 420 430 440 450
FLDSNFTDTE SRDHGASWFK KAFESEPTLQ SGINYAVLLL AAGHQFESSF
460 470 480 490 500
ELRKVGVKLS SLLGKKGNLE KLQSYWEVGF FLGASVLAND HMRVIQASEK
510 520 530 540 550
LFKLKTPAWY LKSIVETILI YKHFVKLTTE QPVAKQELVD FWMDFLVEAT
560 570 580 590 600
KTDVTVVRFP VLILEPTKIY QPSYLSINNE VEEKTISIWH VLPDDKKGIH
610 620 630 640 650
EWNFSASSVR GVSISKFEER CCFLYVLHNS DDFQIYFCTE LHCKKFFEMV
660 670 680 690 700
NTITEEKGRS TEEGDCESDL LEYDYEYDEN GDRVVLGKGT YGIVYAGRDL
710 720 730 740 750
SNQVRIAIKE IPERDSRYSQ PLHEEIALHK HLKHKNIVQY LGSFSENGFI
760 770 780 790 800
KIFMEQVPGG SLSALLRSKW GPLKDNEQTI GFYTKQILEG LKYLHDNQIV
810 820 830 840 850
HRDIKGDNVL INTYSGVLKI SDFGTSKRLA GINPCTETFT GTLQYMAPEI
860 870 880 890 900
IDKGPRGYGK AADIWSLGCT IIEMATGKPP FYELGEPQAA MFKVGMFKVH
910 920 930 940 950
PEIPESMSAE AKAFILKCFE PDPDKRACAN DLLVDEFLKV SSKKKKTQPK
960 970 980 990 1000
LSALSAGSNE YLRSISLPVP VLVEDTSSSS EYGSVSPDTE LKVDPFSFKT
1010 1020 1030 1040 1050
RAKSCGERDV KGIRTLFLGI PDENFEDHSA PPSPEEKDSG FFMLRKDSER
1060 1070 1080 1090 1100
RATLHRILTE DQDKIVRNLM ESLAQGAEEP KLKWEHITTL IASLREFVRS
1110 1120 1130 1140 1150
TDRKIIATTL SKLKLELDFD SHGISQVQVV LFGFQDAVNK VLRNHNIKPH
1160 1170 1180 1190 1200
WMFALDSIIR KAVQTAITIL VPELRPHFSL ASESDTADQE DLDVEDDHEE
1210 1220 1230 1240 1250
QPSNQTVRRP QAVIEDAVAT SGVSTLSSTV SHDSQSAHRS LNVQLGRMKI
1260 1270 1280 1290 1300
ETNRLLEELV RKEKELQALL HRAIEEKDQE IKHLKLKSQP IEIPELPVFH
1310 1320 1330 1340 1350
LNSSGTNTED SELTDWLRVN GADEDTISRF LAEDYTLLDV LYYVTRDDLK
1360 1370 1374
CLRLRGGMLC TLWKAIIDFR NKQT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-849 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K5
Peptide Modified Sequence
AC[+57.0]ANDLLVDEFLK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
928
Peptide - Site of Modification
2
Peptide Start
927
Peptide End
939
CPTAC ID
CPTAC-849
Peptide Molecular Mass
1,506.7388
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 7.7 4.8 6.8 9.7 9.8 10.1 12.4 10.9 12.2 15 15 15
y4 (1+) 14.8 7.4 4.6 10.8 10 9.7 18.3 12.4 10.7 15 15 15
y5 (1+) 6.1 4.7 7.3 12.4 6.1 7.4 13.8 7.7 10.4 15 15 15
y6 (1+) 10.4 3.2 4.8 11.9 10 8.9 15.8 10.5 10.1 15 15 15
y7 (1+) 10.1 7.6 4.4 17.9 13.6 7.8 20.6 15.6 9 15 15 15
y8 (1+) 14.8 9.3 5.5 14.1 8 7.5 20.4 12.3 9.3 15 15 15
y9 (1+) 15 10.1 6 14.2 8.7 8.2 20.7 13.3 10.2 15 15 15
y10 (1+) 13.8 7.3 5.7 10.8 6.5 6.6 17.5 9.8 8.7 15 15 15
y11 (1+) 8.5 2.5 4.2 9.7 8.4 7.8 12.9 8.8 8.9 15 15 15
b3 (1+) 24.9 14.4 7 26.7 12.8 7.2 36.5 19.3 10 15 15 15
sum 4.2 2.3 2.6 7.6 6.8 6 8.7 7.2 6.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-850 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K5
Peptide Sequence
NLYTGKELAAELAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
285
Peptide End
298
CPTAC ID
CPTAC-850
Peptide Molecular Mass
1,547.8308
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 10.4 6.5 7 8.2 5.1 6.7 13.2 8.3 9.7 15 15 15
y4 (1+) 15.5 7.2 11.4 16.4 6.9 10.8 22.6 10 15.7 15 15 15
y5 (1+) 10.4 6 6.8 11.7 5.2 6.9 15.7 7.9 9.7 15 15 15
y6 (1+) 9.7 2.9 2.2 8.5 3.8 2.7 12.9 4.8 3.5 15 15 15
y7 (1+) 7.2 6.2 4.5 7.8 4.2 5 10.6 7.5 6.7 15 15 15
y8 (1+) 7.6 5.1 4.2 8.5 5.3 3.9 11.4 7.4 5.7 15 15 15
y10 (1+) 10.6 5 6.1 10.2 6.5 6.9 14.7 8.2 9.2 15 15 15
sum 4.6 2.6 2.1 4.8 2.3 2.4 6.6 3.5 3.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments